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Methylome Dynamics of Bovine Gametes and in vivo Early Embryos

文献类型： 外文期刊

作者： Duan, Jingyue Ellie 1 ; Jiang, Zongliang Carl 2 ; Alqahtani, Fahad 3 ; Mandoiu, Ion 3 ; Dong, Hong 4 ; Zheng, Xinba 1 ;

作者机构： 1.Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA

2.Louisiana State Univ, AgCtr, Sch Anim Sci, Baton Rouge, LA 70803 USA

3.Univ Connecticut, Dept Comp Sci & Engn, Storrs, CT USA

4.Xinjiang Acad Anim Sci, Inst Anim Sci, Urumqi, Peoples R China

5.Cent Connecticut State Univ, Dept Biol, New Britain, CT 06050 USA

关键词： DNA methylation; gametes; single early embryo; WGBS; bovine

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： DNA methylation undergoes drastic fluctuation during early mammalian embryogenesis. The dynamics of global DNA methylation in bovine embryos, however, have mostly been studied by immunostaining. We adopted the whole genome bisulfite sequencing (WGBS) method to characterize stage-specific genome-wide DNA methylation in bovine sperm, immature oocytes, oocytes matured in vivo and in vitro, as well as in vivo developed single embryos at the 2-, 4-, 8-, and 16-cell stages. We found that the major wave of genome-wide DNA demethylation was complete by the 8-cell stage when de novo methylation became prominent. Sperm and oocytes were differentially methylated in numerous regions (DMRs), which were primarily intergenic, suggesting that these non-coding regions may play important roles in gamete specification. DMRs were also identified between in vivo and in vitro matured oocytes, suggesting environmental effects on epigenetic modifications. In addition, virtually no (less than 1.5%) DNA methylation was found in mitochondrial DNA. Finally, by using RNA-seq data generated from embryos at the same developmental stages, we revealed a weak inverse correlation between gene expression and promoter methylation. This comprehensive analysis provides insight into the critical features of the bovine embryo methylome, and serves as an important reference for embryos produced in vitro, such as by in vitro fertilization and cloning. Lastly, these data can also provide a model for the epigenetic dynamics in human early embryos.

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