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Identification and analysis of microRNAs-mRNAs pairs associated with nutritional status in seasonal sheep

文献类型： 外文期刊

作者： Yang, Heng 1 ; Liu, Xianxia 1 ; Hu, Guangdong 1 ; Xie, Yifan 1 ; Lin, Shan 1 ; Zhao, Zongsheng 1 ; Chen, Jingbo 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Peoples R China

2.Xinjiang Acad Anim Sci, Urumqi 830011, Peoples R China

关键词： Reproduction axis; miRNAs; Target genes; Negative feedback loop

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2018 年 499 卷 2 期

页码：

收录情况： SCI

摘要： Given the important role of nutritional status for reproductive performance, we aimed to explore the potential microRNA (miRNA)-mRNA pairs and their regulatory roles associated with nutritional status in seasonal reproducing sheep. Individual ewes were treated with and without 03 kg/day concentrates, and the body condition score, estrus rate, and related miRNAs and target genes were compared. A total of 261 differentially expressed miRNAs were identified, including 148 hypothalamus-expressed miRNAs and 113 ovary-expressed miRNAs, and 349 target genes were predicted to be associated with nutritional status and seasonal reproduction in sheep. Ultimately, the miR-200b-GNAQ pair was screened and validated as differentially expressed, and a dual luciferase reporter assay showed that miR-200b could bind to the 3'-untranslated region of GNAQ to mediate the hypothalamic-pituitary-ovarian axis. Thus, miR-200b and its target gene GNAQ likely represent an important negative feedback loop, providing a link between nutritional status and seasonal reproduction in sheep toward enhancing reproductive performance and productivity. (C) 2018 Published by Elsevier Inc.

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