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Nutritional status affects the microRNA profile of the hypothalamus of female sheep

文献类型： 外文期刊

作者： Yang, Heng 1 ; Lin, Shan 2 ; Lei, Xiaoping 1 ; Yuan, Cong 1 ; Yu, Yaosheng 1 ; Zhao, Zongsheng 1 ; Chen, Jingbo 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, North Fourth Rd, Shihezi 832003, Peoples R China

2.Shihezi Univ, Coll Life Sci, North Fourth Rd, Shihezi 832003, Peoples R China

3.Xinjiang Acad Anim Sci, Karamay St 151, Urumqi 830000, Peoples R China

关键词： miRNA; oestrus regulation; seasonal breeding

期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：2.311； 五年影响因子：2.396 ）

ISSN： 1031-3613

年卷期： 2018 年 30 卷 7 期

页码：

收录情况： SCI

摘要： Recent studies on the seasonal regulation of the oestrous cycle in sheep have focussed mainly on the responses to photoperiod. However, the brain systems that control reproductive activity also respond to nutritional inputs, although the molecular mechanisms involved are not completely understood. One possibility is that small, non-coding RNAs, such as micro-RNAs (miRNAs), have significant influence. In the present study, the amounts and characteristics of miRNAs in hypothalamus from oestrous and anestrous ewes, fed low-or high-nutrient diets, were compared using Illumina HiSeq sequencing technology. In total, 398 miRNAs, including 261 novel miRNAs, were identified in ewes with an enhanced nutritional status (HEN), whereas 384 miRNAs, including 247 novel miRNAs, were identified in the ewes with a lesser nutritional status (HAN). There were eight conserved and 140 novel miRNAs expressed differentially between the two libraries. Based on quantitative real-time polymerase chain reaction, six miRNAs were assessed to verify the accuracy of the library database. Moreover, the correlation between the miRNA target and several upstream and downstream genes in the oestrus-related pathways were also verified in hypothalamus nerve cells. According to the results, nutritional status plays an important role in oestrous regulation in sheep, and the hypothalamic processes and pathways induced by nutritional signals (folic acid and tyrosine) are different from those induced by photoperiodic regulation of oestrus. We have expanded the repertoire of sheep miRNAs that could contribute to the molecular mechanisms that regulate the initiation of oestrous cycles in anestrous ewes in response to the influence of nutritional status.

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