Acaricidal Activity and Field Efficacy Analysis of the Potential Biocontrol Agent Bacillus vallismortis NBIF-001 against Spider Mites
文献类型: 外文期刊
作者: Zhu, Lei 1 ; Chen, Ling 1 ; Min, Yong 1 ; Liu, Fang 1 ; Liao, Xianqing 1 ; Rao, Ben 1 ; Qiu, Yimin 1 ; Chen, Wei 1 ; Wang, Kaimei 1 ; Yang, Ziwen 1 ; Zhou, Ronghua 1 ; Gong, Yan 1 ; Liu, Xiaoyan 1 ;
作者机构: 1.Natl Biopesticide Engn Technol Res Ctr, Wuhan 430064, Peoples R China
2.Hubei Acad Agr Sci, Hubei Biopesticide Engn Res Ctr, Wuhan 430064, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Microbial Pesticides, Wuhan 430064, Peoples R China
4.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
关键词: spider mites; Bacillus vallismortis; acaricidal activity; field efficacy; protein BVP8; biocontrol
期刊名称:MICROORGANISMS ( 影响因子:4.926; 五年影响因子:5.143 )
ISSN:
年卷期: 2022 年 10 卷 9 期
页码:
收录情况: SCI
摘要: In recent years, spider mites have caused considerable economic losses to global agriculture. However, currently available management strategies are limited because of the rapid development of resistance. In this study, Bacillus vallismortis NBIF-001 was isolated and evaluated for its acaricidal activity. NBIF-001 exhibited a significant lethal effect on spider mites within 48 h. The median lethal concentration (LC50) of the culture powders (3.2 x 10(10) CFU/g) was 50.2 mu g/mL for Tetranychus urticae (red form), 18.0 mu g/mL for T. urticae (green form), and 15.7 mu g/mL for Panonychus citri (McGregor). Cultivation optimisation experiments showed that when the number of spores increased, fermentation toxicity also increased. Moreover, field experiments demonstrated that NBIF-001 performed well in the biocontrol of P. citri, which showed a similar corrected field efficacy with the chemical control (67.1 +/- 7.9% and 71.1 +/- 6.4% after 14 days). Genomics analysis showed that NBIF-001 contains 231 factors and seven gene clusters of metabolites that may be involved in its acaricidal activity. Further bioassays of the fermentation supernatants showed that 50x dilution treatments killed 72.5 +/- 5.4% of the mites in 48 h, which was similar with those of the broth. Bioassays of the supernatant proteins confirmed that various proteins exhibited acaricidal activity. Five candidate proteins were expressed and purified successfully. The bioassays showed that the small protein BVP8 exhibited significant acaricidal activity with an LC50 of 12.4 mu g/mL (T. urticae). Overall, these findings suggest that B. vallismortis NBIF-001 is a potential biocontrol agent for spider mite management.
- 相关文献
作者其他论文 更多>>
-
Preparation of granite powder-based geopolymer by synergistic action of calcination and phosphoric acid
作者:Jia, Luxia;Huang, Min;Huang, Muyang;Luo, Yongpeng;Zhang, Xiuze;Bao, Shenxu;Huang, Min;Bao, Shenxu;Liao, Xianqing;Bao, Shenxu
关键词:calcination; geopolymer; granite powder; phosphoric acid; pore structure
-
Secondary Metabolites from Aspergillus sparsus NBERC_28952 and Their Herbicidal Activities
作者:Wu, Zhaoyuan;Liu, Fang;Ke, Shaoyong;Zhang, Zhigang;Hu, Hongtao;Fang, Wei;Zhang, Yani;Wang, Yueying;Wang, Kaimei;Xiao, Shaoyujia
关键词:Aspergillus; Aspergillus sparsus NBERC_28952; Aspersparins; sydonic acid; herbicidal activities; Echinochloa crusgalli; Amaranthus retroflexus
-
Highly efficient construction of multi-substituted aminopyrazoles derivatives via iodine-mediated three-components reaction as potential anticancer agents
作者:Gao, Zilin;Liu, Manli;Shi, Liqiao;Zhang, Zhigang;Yang, Fei;Wang, Kaimei;Ke, Shaoyong;Gao, Zilin;Chen, Yu;Cao, Xiufang;Huang, Wenbo;Yang, Fei;Cao, Xiufang;Wang, Kaimei;Ke, Shaoyong
关键词:Aminipyrazole derivatives; three-component reaction; reaction mechanism; biological application; SARs; kinase inhibitor; molecular docking
-
Napyradiomycin A4 and Its Relate Compounds, a New Anti-PRV Agent and Their Antibacterial Activities, from Streptomyces kebangsaanensis WS-68302
作者:Zhang, Yani;Fang, Wei;Wang, Kaimei;Zhang, Zhigang;Wu, Zhaoyuan;Shi, Liqiao;Liu, Fang;Wan, Zhongyi;Liu, Manli
关键词:napyradiomycins; anti-PRV; antibacterial; Streptomyces kebangsaanensis WS-68302
-
Antiviral Activity of Benzoheterocyclic Compounds from Soil-Derived Streptomyces jiujiangensis NBERC-24992
作者:Liu, Manli;Zhang, Yani;Wan, Zhongyi;Wang, Yueyin;Wu, Zhaoyuan;Wang, Kaimei;Fang, Wei;Ren, Mengyao;Yang, Xiliang
关键词:heterocyclic; virantmycin; PRV; Streptomyces; antiviral activity
-
Waste plastic to energy storage materials: a state-of-the-art review
作者:Tang, Guoqiang;Qiao, Wenyuan;Wang, Zheng;Liu, Fang;Wu, Hongqu;Tang, Guoqiang;Qiao, Wenyuan;Wang, Zheng;He, Liang;Liu, Minghao;Liu, Changhui;Huang, Wenbo
关键词:
-
Novel Steroidal[17,16-d]pyrimidines Derived from Epiandrosterone and Androsterone: Synthesis, Characterization and Configuration-Activity Relationships
作者:Yang, Fei;Yang, Ziwen;Yang, Fei;Liu, Fang;Min, Yong;Shi, Liqiao;Liu, Manli;Wang, Kaimei;Ke, Shaoyong;Gong, Yan;Yang, Ziwen
关键词:epiandrosterone; androsterone; steroidal[17; 16-d]pyrimidines; synthesis; bioactivity; molecular docking; SARs