Analysis of miRNAs and their target genes associated with mucosal damage caused by transport stress in the mallard duck intestine
文献类型: 外文期刊
作者: Zhang, Hao 1 ; Chen, Fang 1 ; Liang, Zhenhua 1 ; Wu, Yan 1 ; Pi, Jinsong 1 ; Wang, Lixia 2 ; Du, Jinping 1 ; Shen, Jie 1 ; P 1 ;
作者机构: 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan, Peoples R China
2.Wuhan Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan, Peoples R China
期刊名称:PLOS ONE ( 影响因子:3.24; 五年影响因子:3.788 )
ISSN: 1932-6203
年卷期: 2020 年 15 卷 8 期
页码:
收录情况: SCI
摘要: Bowel health is an important factor for duck rearing that has been linked to feed uptake and growth and death rates. Because the regulatory networks associated with acute stress-mediated injury in the duck gastrointestinal tract have not clearly elucidated, we aimed to explore potential miRNA-mRNA pairs and their regulatory roles in oxidative stress injury caused by transport stress. Here, 1-day-old mallard ducklings from the same breeder flock were collected and transported for 8 h, whereas the control group was not being transported. Various parameters reflecting oxidative stress and the tissue appearance of the intestine were assessed. The data showed that the plasma T-AOC and SOD concentrations were decreased in the transported ducklings. The intestine of the transported ducklings also displayed significant damage. High-throughput sequencing of the intestine revealed 44 differentially expressed miRNAs and 75 differentially expressed genes, which constituted 344 miRNA-mRNA pairs. KEGG pathway analysis revealed that the metabolic, FoxO signaling, influenza A and TGF-beta signaling pathways were mainly involved in the mechanism underlying the induction of intestinal damage induced by simulated transport stress in ducks. A miRNA-mRNA pair, miR-217-5p/CHRDL1, was selected to validate the miRNA-mRNA negative relationship, and the results showed that miR-217-5p could influenceCHRDL1expression. This study provides new useful information for future research on the regulatory network associated with mucosal damage in the duck intestine.
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