Transcriptome and metabolome analyses reveal novel insights into the seed germination of Michelia chapensis, an endangered species in China
文献类型: 外文期刊
作者: Zhou, Wuxian 1 ; Duan, Yuanyuan 1 ; Jiang, Xiaogang 1 ; Tan, Xuhui 1 ; Li, Qin 1 ; Wang, Hua 1 ; Zhang, Yajuan 3 ; Zhang, Meide 1 ;
作者机构: 1.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Biol & Cultivat Herb Med, Enshi, Peoples R China
3.Agr & Rural Bur Enshi Tujia & Miao Autonomous Pref, Enshi, Peoples R China
关键词: Michelia chapensis; Seed germination; Plant hormone signal transduction; Starch and sucrose metabolism; Energy supply; Biosynthesis of secondary metabolites
期刊名称:PLANT SCIENCE ( 影响因子:5.2; 五年影响因子:5.7 )
ISSN: 0168-9452
年卷期: 2023 年 328 卷
页码:
收录情况: SCI
摘要: Michelia chapensis Dandy, a well-known medicinal woody plant endemic to China, is endangered and seriously constricted by seed dormancy-induced low-regeneration in natural conditions. Cold stratification can effectively reduce seed dormancy and promote the seed germination of M. chapensis. However, the molecular events and systematic changes that occurred during seed germination in M. chapensis remain largely unknown. In this study, we carried out transcriptomic and metabolomic analyses to elucidate the potential molecular mechanisms un-derlying seed germination in M. chapensis under cold stratification. The results showed that the embryo cells became bigger and looser with increasing stratification time. Moreover, the endosperm appeared reduced due to the consumption of nutrients. Seventeen phytohormones were examined by the metabolome targeted for hor-mones. Compared with the ES (no stratification), the levels of indole-3-acetic acid (IAA) and gibberellin A3 (GA3) were increased in the MS (stratification for 45 days), while the abscisic acid (ABA) was downregulated in both MS and LS (stratification for 90 days). The transcriptome profiling identified 24975 differentially expressed genes (DEGs) in the seeds during germination. The seed germination of M. chapensis was mainly regulated by the biological pathways of plant hormone signal transduction, energy supply, secondary metabolite biosynthesis, photosynthesis-related metabolism, and transcriptional regulation. This study reveals the biological evidence of seed germination at the transcriptional level and provides a foundation for unraveling molecular mechanisms regulating the seed germination of M. chapensis.
- 相关文献
作者其他论文 更多>>
-
First specific detection and validation of tomato wilt caused by Fusarium brachygibbosum using a PCR assay
作者:Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Liu, Jun;Wang, Hua;Liu, Quanke
关键词:Tomato wilt; Fusarium brachygibbosum; Identification; Specificity; Sensitivity; Whole genome sequence comparison
-
TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.
作者:Jiang, Xiaogang;Zhou, Wuxian;Wang, Hua;You, Jinwen;Zhang, Meide;Liu, Wenlu
关键词:Codonopsis tangshen Oliv; proteome; continuous cropping; biological process
-
Transcriptomic and metabolomic analyses provide new insights into the appropriate harvest period in regenerated bulbs of Fritillaria hupehensis
作者:Duan, Yuanyuan;Wu, Jiaqi;Wang, Fanfan;Zhang, Kaiqi;Guo, Xiaoliang;Tang, Tao;Mu, Sen;You, Jingmao;Guo, Jie;Duan, Yuanyuan;Guo, Jie;Duan, Yuanyuan;Wang, Fanfan;Tang, Tao;You, Jingmao
关键词:Fritillaria hupehensis; bulb development; steroidal alkaloids; metabolic; WGCNA
-
Synthesis, antifungal activity and mechanism of action of novel chalcone derivatives containing 1,2,4-triazolo-[3,4-b]-1,3,4-thiadiazole
作者:Zhan, Wenliang;Zhou, Ran;Mao, Piao;Yuan, Chunmei;Zhang, Tao;Liu, Yi;Tian, Jiao;Xue, Wei;Zhan, Wenliang;Zhou, Ran;Mao, Piao;Yuan, Chunmei;Zhang, Tao;Liu, Yi;Tian, Jiao;Xue, Wei;Zhan, Wenliang;Zhou, Ran;Mao, Piao;Yuan, Chunmei;Zhang, Tao;Liu, Yi;Tian, Jiao;Xue, Wei;Wang, Hua
关键词:Chalcone; 1,2,4-triazolo-[3,4-b]-1,3,4-thiadiazole; Antifungal activity; Mechanism study
-
Survival of Pseudomonas syringae pv. actinidiae in detached kiwifruit leaves at different environmental conditions
作者:Deng, Siyi;Chang, Wei;Que, Yawei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Que, Yawei;Liu, Jun;Wang, Hua;Deng, Siyi;Chang, Wei;Que, Yawei;Liu, Jun;Wang, Hua
关键词:Kiwifruit canker; Pseudomonas syring pv; actinidiae; Infection path; Survival time; Population growth; Temperature and humidity
-
Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen
作者:Jiang, Xiaogang;Zhou, Wuxian;Wang, Hua;You, Jinwen;Zhang, Meide;Wassie, Misganaw;Ma, Guangjing
关键词:Codonopsis tangshen; heat-stress; heat-responsive genes; RNA-sequencing; transcriptome
-
Coptischinensis Franch root rot infection disrupts microecological balance of rhizosphere soil and endophytic microbiomes
作者:Tang, Tao;Wang, Fanfan;Mao, Ting;Guo, Jie;Guo, Xiaoliang;You, Jingmao;Tang, Tao;Wang, Fanfan;Guo, Jie;Duan, Yuanyuan;You, Jingmao;Fang, Guobin;Kuang, Hui;Sun, Guangzhong
关键词:Coptis chinensis; rhizosphere soil and endophytic microbiomes; microecological regulation; microecological balance; soil physicochemical factors