文献类型: 外文期刊
作者: Chen, Ting 1 ; Ma, Fei 2 ; Peng, Yongjia 2 ; Sun, Ruiping 3 ; Xi, Qianyun 1 ; Sun, Jiajie 1 ; Zhang, Jin 2 ; Zhang, Yongliang 1 ; Li, Meng 2 ;
作者机构: 1.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Natl Engn Res Ctr Breeding Swine Ind, Coll Anim Sci,Guangdong Prov Key Lab Anim Nutr Co, 483 Wushan Rd, Guangzhou 510642, Peoples R China
2.Jiaxing Univ, Coll Biol Chem Sci & Engn, Jiaxing 314000, Peoples R China
3.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Hainan, Peoples R China
关键词: Exogenous; miRNA; Cross-kingdom; Adipogenesis
期刊名称:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL ( 影响因子:2.723; 五年影响因子:2.303 )
ISSN: 1071-2690
年卷期: 2022 年 58 卷 6 期
页码:
收录情况: SCI
摘要: Adipogenesis is important in the development of fat deposition. Evidence showed that plant microRNAs (miRNAs) could be absorbed by the digestive tract and exert regulatory effects on animals' physiological processes. However, the regulation of plant miRNA on host lipogenesis remains unknown. This study explored the potential function of plant miRNA, miR167e-5p, in adipogenesis in vitro. The presentation of plant miR167e-5p improved lipid accumulation in 3T3-L1 cells. Bioinformatics prediction and luciferase reporter assay indicated that miR167e-5p targeted beta-catenin. MiR167e-5p could not only negatively affect the expression of beta-catenin but also showed a positive effect on several fat synthesis-related genes, peroxisome proliferator-activated receptor gamma (Ppary), CCAAT/enhancer-binding protein alpha (Cebp alpha), fatty acid-binding protein 4 (Ap2), lipolysis genes, adipose triglyceride lipase (Atgl), and hormone-sensitive lipase (Hsl) messenger RNA levels. Meanwhile, lipid accumulation and the expression of the beta-catenin and other five fat synthesis-related genes were recovered to their original pattern by adding the miR167e-5p inhibitor in 3T3-L1 cells. The immunoblot confirmed the same expression pattern in protein levels in beta-catenin, PPAR-gamma, FAS, and HSL. This research demonstrates that plant miR167e-5p can potentially affect adipogenesis through the regulation of beta-catenin, suggesting that plant miRNAs could be a new class of bioactive ingredients in adipogenesis.
- 相关文献
作者其他论文 更多>>
-
Effects of dietary supplementation with dandelion tannins or soybean isoflavones on growth performance, antioxidant function, intestinal morphology, and microbiota composition in Wenchang chickens
作者:Li, Xiang;Sun, Ruiping;Ou, Yangkun;Qi, Qi;Xie, Yali;Wei, Limin;Li, Xiang;Gong, Yuanfang;Liu, Quanwei;Zhao, Guiping;Wei, Limin;Wang, Xiuping;Hu, Chenjun;Jiang, Shouqun
关键词:growth performance; biochemical blood indexes; antioxidant function; intestinal health; dandelion tannin; soybean isoflavones; Wenchang chicken
-
Novel Haplotype in the HHEX Gene Promoter Associated with Body Length in Pigs
作者:Luo, Yabiao;Xu, Qiao;Xue, Mingming;Wang, Yubei;Yang, Xiaoyang;Chan, Shuheng;Tang, Qiguo;Fang, Meiying;Xu, Qiao;Wang, Feng;Sun, Ruiping;Chao, Zhe;Fang, Meiying
关键词:HHEX; body length; expression pattern; gene polymorphism; promoter activity
-
MiR-143 Targets SYK to Regulate NEFA Uptake and Contribute to Thermogenesis in Male Mice
作者:Liu, Jie;Chen, Ting;Wang, Huan;Luo, Junyi;Chen, Xingping;Jiang, Qingyan;Xi, Qianyun;Sun, Jiajie;Zhang, Lin;Zhang, Yongliang;Liu, Jie;Wei, Limin;Liu, Jie;Wei, Limin;Chen, Xingping
关键词:BAT; WAT; thermogenesis; miR-143; AC9; SYK
-
Dietary Supplementation with Putrescine Improves Growth Performance and Meat Quality of Wenchang Chickens
作者:Qi, Qi;Zhao, Guiping;Wei, Limin;Qi, Qi;Sun, Ruiping;Liu, Quanwei;Ouyang, Kun;Xie, Yali;Li, Xiang;Wu, Wei;Liu, Yuhang;Wei, Limin;Hu, Chengjun;Zhang, Haojie
关键词:amino acid; fatty acid; growth performance; putrescine; Wenchang chickens
-
Interactive effects of high temperature and crude protein levels on growth performance, nitrogen excretion, and fecal characteristics of broilers
作者:Liu, Quanwei;Wei, Limin;Zheng, Xinli;Sun, Ruiping;Liu, Quanwei;Wei, Limin;Sun, Ruiping;Feng, Jinghai;Zhang, Minhong;Hu, Chengjun
关键词:High temperature; Crude protein level; Broiler; Nitrogen excretion; Fecal characteristics; Growth performance
-
MiR-125b-2 knockout increases high-fat diet-induced fat accumulation and insulin resistance
作者:Wei, Li-Min;Sun, Rui-Ping;Dong, Tao;Liu, Jie;Chen, Ting;Zeng, Bin;Wu, Jia-Han;Luo, Jun-yi;Sun, Jia-Jie;Xi, Qian-Yun;Zhang, Yong-Liang;Wei, Li-Min;Sun, Rui-Ping
关键词:
-
Oral Administration of Bovine and Porcine Milk Exosome Alter miRNAs Profiles in Piglet Serum
作者:Lin, Delin;Chen, Ting;Xie, Meiying;Li, Meng;Zeng, Bin;Zhu, Yanling;Ye, Dingze;Wu, Jiahan;Sun, Jiajie;Xi, Qianyun;Jiang, Qingyan;Zhang, Yongliang;Sun, Ruiping
关键词: