文献类型： 外文期刊

作者： Hou, Yujun ¹ ; Wong, Darren C. J. ⁴ ; Li, Qingyun ¹ ; Zhou, Huimin ¹ ; Zhu, Zhenfei ¹ ; Gong, Linzhong ⁵ ; Liang, Ju ⁶ ; Ren, Hongsong ⁶ ; Liang, Zhenchang ⁷ ; Wang, Qingfeng ¹ ; Xin, Haiping ¹ ;

作者机构： 1.Chinese Acad Sci, Ctr Econ Bot, Key Lab Plant Germplasm Enhancement & Specialty A, Core Bot Gardens,Wuhan Bot Garden, Wuhan 430074, Peoples R China

2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia

5.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430064, Peoples R China

6.Xinjiang Acad Agr Sci, Turpan Inst Agr Sci, Urumqi 830091, Xinjiang, Peoples R China

7.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China

8.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China

关键词： Chilling stress; Ethylene; Phytohormone; Transcriptomics; Grapevines

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 196 卷

页码：

收录情况： SCI

摘要： Ethylene (ETH) plays important roles in various development programs and stress responses in plants. In grapevines, ETH increased dramatically under chilling stress and is known to positively regulate cold tolerance. However, the role of ETH in transcriptional regulation during chilling stress of grapevine leaves is still not clear. To address this gap, targeted hormone profiling and transcriptomic analysis were performed on leaves of Vitis amurensis under chilling stress with and without aminoethoxyvinylglycine (AVG, a inhibitor of ETH synthesis) treatment. APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) and WRKY transcription factors (TF) were only the two highly enriched TF families that were consistently up-regulated during chilling stress but inhibited by AVG. The comparison of leaf transcriptomes between chilling treatment and chilling with AVG allowed the identification of potential ETH-regulated genes. Potential genes that are positively regulated by ETH are enriched in solute transport, protein biosynthesis, phytohormone action, antioxidant and carbohydrate metabolism. Conversely, genes related to the synthesis and signaling of ETH, indole-3-acetic acid (IAA), abscisic acid (ABA) were up-regulated by chilling treatment but inhibited by AVG. The contents of ETH, ABA and IAA also paralleled with the transcriptome data, which suggests that the response of ABA and IAA during chilling stress may regulate by ETH signaling, and together may belong to an integrated network of hormonal signaling pathways under-pinning chilling stress response in grapevine leaves. Together, these findings provide new clues for further studying the complex regulatory mechanism of ETH under low-temperature stress in plants more generally and new opportunities for breeding cold-resilient grapevines.