文献类型： 外文期刊

作者： Zhou, Yong ¹ ; Han, Li-Rong ¹ ; He, Hong-Wei ¹ ; Sang, Bu ³ ; Yu, Dai-Lin ³ ; Feng, Jun-Tao ¹ ; Zhang, Xing ¹ ;

作者机构： 1.Northwest Agr & Forestry Univ, Res & Dev Ctr Biorat Pesticides, Yangling 712100, Shaanxi, Peoples R China

2.Shaanxi Res Ctr Biopesticide Engn & Technol, Yangling 712100, Shaanxi, Peoples R China

3.Tibet Acad Agr & Anim Husb Sci, Agr Res Inst, Lhasa 850032, Tibet, Peoples R China

关键词： scale-up;glycoprotein;Streptomyces;anti-TMV;volumetric oxygen transfer coefficient

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2018 年 23 卷 1 期

页码：

收录情况： SCI

摘要： The effects of temperature, agitation and aeration on glycoprotein GP-1 production by Streptomyces kanasenisi ZX01 in bench-scale fermentors were systematically investigated. The maximum final GP-1 production was achieved at an agitation speed of 200 rpm, aeration rate of 2.0 vvm and temperature of 30 degrees C. By using a dynamic gassing out method, the effects of agitation and aeration on volumetric oxygen transfer coefficient (k(L)a) were also studied. The values of volumetric oxygen transfer coefficient in the logarithmic phase increased with increase of agitation speed (from 14.53 to 32.82 h(-1)) and aeration rate (from 13.21 to 22.43 h(-1)). In addition, a successful scale-up from bench-scale to pilot-scale was performed based on volumetric oxygen transfer coefficient, resulting in final GP-1 production of 3.92, 4.03, 3.82 and 4.20 mg/L in 5 L, 15 L, 70 L and 500 L fermentors, respectively. These results indicated that constant volumetric oxygen transfer coefficient was appropriate for the scale-up of batch fermentation of glycoprotein GP-1 by Streptomyces kanasenisi ZX01, and this scale-up strategy successfully achieved 100-fold scale-up from bench-scale to pilot-scale fermentor.