文献类型： 外文期刊

作者： Zhong, Yiwang ¹ ; Wen, Ke ¹ ; Li, Xingang ¹ ; Wang, Shasha ¹ ; Li, Sansan ¹ ; Zeng, Yuhong ¹ ; Cheng, Yanbo ¹ ; Ma, Qibin ¹ ; Nian, Hai ¹ ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Guangdong, Peoples R China

2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Guangdong, Peoples R China

3.South China Agr Univ, Coll Agr, Guangdong Subctr Natl Ctr Soybean Improvement, Guangzhou 510642, Guangdong, Peoples R China

4.Hainan Acad Agr Sci, Vegetable Res Inst, Key Lab Vegetable Biol Hainan Prov, Haikou 570228, Hainan, Peoples R China

5.Chinese Acad Sci, Inst Genet & Dev Biol, Hainan Yazhou Bay Seed Lab, Sanya 572025, Hainan, Peoples R China

6.Hainan Univ, Sanya Nanfan Res Inst, Hainan Yazhou Bay Seed Lab, Sanya 572025, Hainan, Peoples R China

关键词： soybean; sulfur-containing amino acids; protein; QTL

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 1 期

页码：

收录情况： SCI

摘要： Soybean is a major source of high-quality protein for humans and animals. The content of sulfur-containing amino acids (SAA) in soybean is insufficient, which has become the main factor limiting soybean nutrition. In this study, we used the high-density genetic maps derived from Guizao 1 and Brazil 13 to evaluate the quantitative trait loci of cysteine (Cys), methionine (Met), SAA, glycinin (7S), beta-conglycinin (11S), ratio of glycinin to beta-conglycinin (RGC), and protein content (PC). In genetic map linkage analysis, the major and stable 44 QTLs were detected, which shared nine bin intervals. Among them, the bin interval (bin157-bin160) on chromosome 5 was detected in multiple environments as a stable QTL, which was linked to 11S, 7S, RGC, and SSA. Based on the analysis of bioinformatics and RNA-sequencing data, 16 differentially expressed genes (DEGs) within these QTLs were selected as candidate genes. These results will help to elucidate the genetic mechanism of soybean SAA-related traits and provide the basis for the gene mining of sulfur-containing amino acids.