Phosphate-triggered ratiometric multicolor immunosensor based on nanobody-alkaline phosphatase fusion protein for sensitive detection of fenitrothion
文献类型： 外文期刊
作者： Liu, Min -Ling 1 ; Zeng, Xi 2 ; Deng, Hao 3 ; Wang, Yu 2 ; Zhang, Yi-Feng 1 ; Shen, Yu-Dong 1 ; Luo, Lin 1 ; Wang, Hong 1 ; Chen, Zi-Jian 1 ; Xu, Zhen-Lin 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China
2.Guangzhou Inst Food Inspect, Guangzhou 510410, Peoples R China
3.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold Chain Hainan P, Haikou 570100, Peoples R China
关键词： Multicolor immunosensor; Ratiometric assay; Nanobody-alkaline phosphase fusion; Fenitrothion
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：9.221； 五年影响因子：7.676 ）
ISSN：
年卷期： 2022 年 373 卷
页码：
收录情况： SCI
摘要： Multicolor immunosensor is a promising tool for on-site rapid analysis by naked eye without bulky instruments. Herein, a multicolor immunosensor employed a specific nanobody-alkaline phosphatase fusion protein (VHHjd8-ALP) for biosensing of fenitrothion was developed. After one-step indirect competitive immunoassay, the binding VHHjd8-ALP in microplate catalyzed ascorbyl-2-phosphate (AAP) into ascorbic acid, which propose dual readout including multicolor ratiometric visible immunoassay (MRVIA) and multicolor ratiometric fluoroimmunoassay (MRFIA). For MRVIA, K3[Fe(CN)6] and Fe3+ was employed for multicolor generation. The substrate AAP can chelate with Fe3+ to form orange AAP-Fe3+ complex. When ALP catalyzed AAP into ascorbic acid, the latter can reduced K3[Fe(CN)6] into K4[Fe(CN)6], which reacted with Fe3+ to produced Prussian blue via self-assembly. A visible color transition from orange to blue against fenitrothion was obtained. For MRFIA, the generated ascorbic acid etched MnO2 nanosheet to control the concentration of Na2SO3, which can convert 9-anthraldehyde into sulfuretted anthracene accompanied by a fluorescent color transition from green to blue. These two methods showed visible multicolor transition against fenitrothion for naked-eye analysis. The recovery and blind samples test verified good accuracy and practicability, which can be used for rapid screening of fenitrothion by a naked -eye semiquantitative analysis mode.
- 相关文献
作者其他论文 更多>>
-
Development of a Biotinylated Nanobody-Based Gold Nanoparticle Immunochromatographic Assay for the Detection of Procymidone in Crops
作者：Liu, Min-Ling;He, Xiao-Ting;Xu, Zhen-Lin;Shen, Yu-Dong;Luo, Lin;Shen, Xing;Wang, Hong;Deng, Hao;Hammock, Bruce;Hammock, Bruce;Chen, Zi-Jian
关键词：procymidone; nanobodies; biotinylation; streptavidin; immunochromatographic assay
-
Highly selective monoclonal antibody-based fluorescence immunochromatographic assay for the detection of fenpropathrin in vegetable and fruit samples
作者：Xu, Zi-Hong;Wang, Jun-Kai;Chen, Run-Xi;Huang, Wei;Lei, Hong-Tao;Xu, Zhen-Lin;Luo, Lin;Ye, Qiu-Xiong;Jiang, Lin-Feng;Deng, Hao
关键词：
-
Comparative nutritional and metabolic analysis reveals the taste variations during yellow rambutan fruit maturation
作者：Deng, Hao;Wu, Guang;Zhang, Ronghu;Xu, Bin;Zhou, Liying;Deng, Hao;Wu, Guang;Zhang, Ronghu;Deng, Hao;Wu, Guang;Zhang, Ronghu;Chen, Zhe;Yin, Qingchun;Chen, Zhe;Chen, Zhe
关键词：Rambutan; Nutrition; BY2; Maturation; Widely targeted metabolomics
-
Metabolic Profiling and Potential Taste Biomarkers of Two Rambutans during Maturation
作者：Deng, Hao;Wu, Guang;Guo, Li;Zhou, Liying;Xu, Bin;Deng, Hao;Wu, Guang;Deng, Hao;Wu, Guang;Hu, Fuchu;Chen, Zhe;Hu, Fuchu;Chen, Zhe;Yin, Qingchun
关键词：rambutan; sugar; acid; biomarker; widely targeted metabolomics; maturation
-
Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan
作者：Wu, Guang;Guo, Li;Deng, Hao;Wu, Guang;Deng, Hao;Wu, Guang;Deng, Hao;Zhou, Chuang;Yang, Ziming;Yin, Qingchun;Deng, Hao
关键词：Carboxymethyl chitosan; ClO2 preservative; Longan; Preparation; Evaluation
-
Structural variation and introgression from wild populations in East Asian cattle genomes confer adaptation to local environment
作者：Xia, Xiaoting;Zhang, Fengwei;Li, Shuang;Luo, Xiaoyu;Dong, Zheng;Wang, Shikang;Jin, Liangliang;Dang, Ruihua;Huang, Yongzhen;Lan, Xianyong;Ren, Gang;Wang, Yu;Cheng, Haijian;Chen, Hong;Pang, Weijun;Lei, Chuzhao;Chen, Ningbo;Peng, Lixin;Pausch, Hubert;Leonard, Alexander S.;Crysnanto, Danang;Tong, Bin;Lenstra, Johannes A.;Han, Jianlin;Han, Jianlin;Li, Fuyong;Xu, Tieshan;Gu, Lihong;Gao, Yuanpeng;Ma, Zhijie;Cheng, Haijian;Ma, Yun
关键词：Structural variation; Genome assembly; Long-read sequencing; East Asian cattle
-
Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter
作者：Wu, Yueying;Ji, Hengkuan;Li, Caisheng;Hou, Zhengwei;Huang, Cheng;Chen, Liming;Wu, Zhipeng;Wang, Yu;Fu, Chuanliang;Zhang, Dongming;Wang, Yu;Fu, Chuanliang;Zhang, Dongming;Qiu, Yong
关键词：Commercial organic fertilizers; Dissolved organic matter; Molecular weight fractionation; FT-ICR-MS; 2D-COS
