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Identification of an intergenic region that is not essential for replication of goatpox virus

文献类型： 外文期刊

作者： Ma, Wenge 1 ; Wang, Fang 2 ; Yu, Li 2 ; Xue, Ying 1 ; Wang, Yan 1 ; Wei, Jie 1 ; Huang, Jiong 1 ; Fu, Zihua 1 ;

作者机构： 1.Xinjiang Acad Anim Sci, Inst Vet Med, Urumqi 830000, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Livestock Infect Dis, Harbin 150001, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2010 年 155 卷 8 期

页码：

收录情况： SCI

摘要： A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.

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