文献类型： 外文期刊
作者： Yang, Baolin 1 ; Wang, Mingxia 1 ; Liu, Wenming 1 ; Xu, Zhiqiang 1 ; Wang, Haiwei 1 ; Yang, Decheng 2 ; Ma, Wenge; Zho 1 ;
作者机构： 1.Chinese Acad Agr Sci, Div Livestock Infect Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, 678 Haping Rd Xiangfang Dist, Harbin 150069, Heilongjiang, Peoples R China
2.Chinese Acad Agr Sci, Div Livestock Infect Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, 678 Haping Rd Xiangfang Dist, Harbin 150069, Heilo
关键词： Foot-and-mouth disease virus;Monoclonal antibody;VP2;Epitope mapping
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2017 年 162 卷 12 期
页码：
收录情况： SCI
摘要： Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. VP2 is a structural protein of FMDV. In this study, an FMDV serotype-independent monoclonal antibody (MAb), 10B10, against the viral capsid protein VP2 was generated, and a series of GST fusion proteins expressing a truncated peptide of VP2 was subjected to Western blot analysis using MAb 10B10. Their results indicated that the peptide (TLLEDRILT16)-T-8 of VP2 is the minimal requirement of the epitope recognized by MAb 10B10. Importantly, this linear epitope was highly conserved among all seven serotypes of FMDV in a sequence alignment analysis. Subsequent alanine-scanning mutagenesis analysis revealed that the residues Thr(8) and Asp(12) of the epitope were crucial for MAb-10B10 binding. Furthermore, Western blot analysis also revealed that the MAb 10B10-directed epitope could be recognized by positive sera from FMDV-infected cattle. The discovery that MAb 10B10 recognizes a serotype-independent linear epitope of FMDV suggests potential applications for this MAb in the development of serotype-independent tests for FMDV.
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