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Shortened and multivalent aptamers for ultrasensitive and rapid detection of alternariol in wheat using optical waveguide sensors

文献类型： 外文期刊

作者： Wang, Shuo 1 ; Gao, Hualong 1 ; Wei, Zhenzhe 1 ; Zhou, Jianshuo 1 ; Ren, Shang 1 ; He, Junlin 2 ; Luan, Yunxia 3 ; Lou, Xinhui 1 ;

作者机构： 1.Capital Normal Univ, Dept Chem, Xisanhuan North Rd 105, Beijing 100048, Peoples R China

2.Beijing Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Agr Stand & Testing, Agr Prod Qual & Safety Risk Assessment Lab,Dept A, Beijing Municipal Key Lab Agr Environm Monitoring, Middle Rd Shuguang Huayuan 9, Beijing 100097, Peoples R China

关键词： Aptamer; Multivalent; Critical binding site; Alternariol; Aptasensor; Waveguide sensor

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.545； 五年影响因子：10.722 ）

ISSN： 0956-5663

年卷期： 2022 年 196 卷

页码：

收录情况： SCI

摘要： Alternariol (AOH) is one of the common mycotoxins existing in a variety of foods at low level. Aptamers hold great promise for the development of sensitive and rapid aptasensors, but suffer from the excessive length and the difficulty in identification of critical binding domains (CBDs). In this study, the 5 nt CBD of the original 59-nt AOH aptamer (AOH-59, K-D = 423 nM) was identified to be a 'C' bulge in between two A-T base pairs. AOH-59 was successfully shortened to a 23 nt aptamer (AOH 6C, K-D = 701 nM). A 30 nt bivalent aptamer B-2-3 (K-D = 445 nM) and a 39 nt trivalent aptamer T-2-3 (K-D = 274 nM) were obtained by simply incorporating one or two CBDs into AOH 6C. The AOH 6C-, B-2-3-, and T-2-3-based optical waveguide aptasensors possessed the unprecedented detection of limits (LODs, S/N = 3) of 42 +/- 3, 6 +/- 1 and 2 +/- 1 fM, respectively. Using the AOH 6C-based sensor as an example, we further demonstrated the detection of AOH spiked in wheat powder with a LOD of 37 pg/g, 20-230-fold lower than those achieved by ELISAs. The sensor was capable for 35 times 2-min regeneration and the assay time including the extraction of AOH from wheat was only about 1 h. We not only devised the first aptasensors for AOH detection, but also provided a simple strategy to design multivalent aptamers for small molecule targets.

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