您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Three-step evolutionary enhanced capillary electrophoresis-SELEX for aptamer selection of exosome vesicles

文献类型： 外文期刊

作者： Zhao, Liping 1 ; Yang, Ge 3 ; Zhu, Chao 4 ; Li, Linsen 2 ; Zhao, Yi 2 ; Luan, Yunxia 1 ; Qu, Feng 2 ;

作者机构： 1.Inst Qual Stand & Testing Technol BAAFS, Agr Prod Qual & Safety Risk Assessment Lab, Dept Agr, Beijing 100097, Peoples R China

2.Beijing Inst Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, 5 South Zhongguancun St, Beijing 100081, Peoples R China

3.Chinese Acad Med Sci & Peking Union Med Coll, CAMS Key Lab Antiviral Drug Res, Beijing Key Lab Antimicrobial Agents, NHC Key Lab Biotechnol Antibiot,Inst Med Biotechno, Beijing 100050, Peoples R China

4.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China

关键词： NK exosome; CE-SELEX; Selection strategy; Aptamer

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）

ISSN： 0039-9140

年卷期： 2024 年 267 卷

页码：

收录情况： SCI

摘要： As various disease biomarkers, drugs/biomolecules carriers and physiological function regulator, exosomes play significant roles in various physiological and pathological process. Thus, the purification and detection of some exosome are of great significance, which make it necessary for the development of the recognition elements. Aptamers with high affinity and specificity exhibited well in the above areas. In this work, a new three-step evolutionary enhanced strategy based on capillary electrophoresis (CE)-SELEX was proposed for effective aptamer selection of exosome vesicles for the first time. Natural killer cells (NK) exosome was used as the model target. And the aptamer could be obtained in five round CE selection within three steps evolution including precise nucleic acid sequence convergence (with NK exosome specific proteins as targets in round 1st and 3rd), affinity evolution (with NK exosome as target in round 2nd and 4th) and specific evolution (negative selection in round 5th). Arising from the compatibility of CE-SELEX for exosome vesicles aptamer selection, and the proposed strategy, aptamer against NK exosome had been selected with good affinity (KD value of 27.6 nM) and excellent specificity. The as proposed CE-SELEX strategy paves a novel way for recognition element selection of exosome vesicles.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台