Investigation on the Anaphylaxis and Anti-Digestive Stable Peptides Identification of Ultrasound-Treated alpha-Lactalbumin during In-Vitro Gastroduodenal Digestion
文献类型: 外文期刊
作者: Wang, Xumei 1 ; Tu, Zongcai 1 ; Liu, Guangxian 4 ; Wang, Hui 1 ; Hu, Yueming 1 ; Huang, Tao 5 ;
作者机构: 1.Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China
2.Jiangxi Normal Univ, Natl R&D Ctr Freshwater Fish Proc, Nanchang 330022, Jiangxi, Peoples R China
3.Jiangxi Normal Univ, Engn Res Ctr Freshwater Fish High Value Utilizat, Nanchang 330022, Jiangxi, Peoples R China
4.Jiangxi Acad Agr Sci, Inst Food Sci & Technol, Nanchang 330200, Jiangxi, Peoples R China
5.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315800, Peoples R China
关键词: alpha-lactalbumin; ultrasound; in-vitro gastroduodenal digestion; anaphylaxis; anti-digestive stable peptides; high-resolution mass spectrometry
期刊名称:FOODS ( 影响因子:4.35; 五年影响因子:4.957 )
ISSN:
年卷期: 2021 年 10 卷 11 期
页码:
收录情况: SCI
摘要: Our previous studies indicated that ultrasound treatment can increase the anaphylaxis of protein. However, investigation on the anaphylaxis changes of ultrasound-treated alpha-lactalbumin (ALA) during digestion is lacking. The anaphylaxis of ultrasound-treated ALA and its digesta was investigated. The anti-digestive stable peptides were identified by high-resolution mass spectrometry. Ultrasound induced the tertiary structure of ALA to unfold and increased its anaphylaxis. During digestion, the anaphylaxis of both gastric and gastroduodenal digesta was further increased. There are two reasons for this phenomenon. On the one hand, linear epitopes played an important role in affecting anaphylaxis compared with the conformational epitope, and some linear epitopes were still retained on the anti-digestive stable peptides produced after gastroduodenal digestion, resulting in increased anaphylaxis after digestion. On the other hand, the presence of intact ALA molecules after digestion still remained strong anaphylaxis. Compared with the digesta of untreated ALA, the digesta of ultrasound-treated ALA possessed higher anaphylaxis. The results indicated that ultrasound increased the anaphylaxis of ALA during digestion.
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