文献类型： 外文期刊

作者： Wang, Guanglong ¹ ; Tian, Chang ² ; Wang, Yunpeng ¹ ; Wan, Faxiang ¹ ; Hu, Laibao ¹ ; Xiong, Aisheng ³ ; Tian, Jie ⁴ ;

作者机构： 1.Huaiyin Inst Technol, Sch Life Sci & Food Engn, Huaian, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Key Lab Landscape Agr, Minist Agr, Nanjing, Jiangsu, Peoples R China

3.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China

4.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Qinghai Key Lab Vegetable Genet & Physiol, Qinghai Acad Agr & Forestry Sci,Acad Agr & Forest, Xining, Qinghai, Peoples R China

关键词： Reference genes; Garlic; Salt stress; qRT-PCR; Gene expression

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2019 年 7 卷

页码：

收录情况： SCI

摘要： Quantitative real-time reverse-transcriptase PCR (qRT-PCR) has been frequently used for detecting gene expression. To obtain reliable results, selection of suitable reference genes is a fundamental and necessary step. Garlic (Allium sativum), a member from Alliaceae family, has been used both as a food flavoring and as a traditional medicine. In the present study, garlic plants were exposed to salt stress (200 mM NaCl) for 0, 1, 4 and 12 h, and garlic roots, bulbs, and leaves were harvested for subsequent analysis. The expression stability of eight candidate reference genes, eukaryotic translation initiation factor 4 alpha (eIF-4 alpha), actin (ACTIN), tubulin beta-7 (TUB7), TAP42-interacting protein of 41 kDa (TIP41), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), SAND family protein (SAND), elongation factor 1 alpha (EF -1 alpha), and protein phosphatase 2A (PP2A) were evaluated by geNorm, NormFinder, and BestKeeper. All genes tested displayed variable expression profiles under salt stress. In the leaf and root group, ACTIN was the best reference gene for normalizing gene expression. In garlic dove, ACTIN and SAND were the least variable, and were suitable for gene expression studies under salt stress; these two genes also performed well in all samples tested. Based on our results, we recommend that it is essential to use specific reference genes in different situations to obtain accurate results. Using a combination of multiple stable reference genes, such as ACTIN and SAND, to normalize gene expression is encouraged. The results from the study will be beneficial for accurate determination of gene expression in garlic and other plants.