文献类型： 外文期刊

作者： Ma, Xiao ¹ ; Gai, Wen-Xian ¹ ; Qiao, Yi-Ming ¹ ; Ali, Muhammad ¹ ; Wei, Ai-Min ² ; Luo, De-Xu ³ ; Li, Quan-Hui ¹ ; Gong, ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China

2.Tianjin Vegetable Res Ctr, Tianjin 300192, Peoples R China

3.Xuhuai Reg Huaiyin Inst Agr Sci, Huaian 223001, Jiangsu, Peoples R China

4.Qinghai Acad Agr & Forestry Sci, Xining 810016, Qinghai, Peoples R China

5.State Key Lab Vegetable Germplasm Innovat, Tianjin 300384, Peoples R China

关键词： Pepper; Genome-wide; CBL and CIPK family; CaCIPK1; Abiotic stress; Phytophthora capsici

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2019 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background Calcineurin B-like proteins (CBLs) are major Ca2+ sensors that interact with CBL-interacting protein kinases (CIPKs) to regulate growth and development in plants. The CBL-CIPK network is involved in stress response, yet little is understood on how CBL-CIPK function in pepper (Capsicum annuum L.), a staple vegetable crop that is threatened by biotic and abiotic stressors. Results In the present study, nine CaCBL and 26 CaCIPK genes were identified in pepper and the genes were named based on their chromosomal order. Phylogenetic and structural analysis revealed that CaCBL and CaCIPK genes clustered in four and five groups, respectively. Quantitative real-time PCR (qRT-PCR) assays showed that CaCBL and CaCIPK genes were constitutively expressed in different tissues, and their expression patterns were altered when the plant was exposed to Phytophthora capsici, salt and osmotic stress. CaCIPK1 expression changed in response to stress, including exposure to P. capsici, NaCl, mannitol, salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), ethylene (ETH), cold and heat stress. Knocking down CaCIPK1 expression increased the susceptibility of pepper to P. capsici, reduced root activity, and altered the expression of defense related genes. Transient overexpression of CaCIPK1 enhanced H2O2 accumulation, cell death, and expression of genes involved in defense. Conclusions Nine CaCBL and 26 CaCIPK genes were identified in the pepper genome, and the expression of most CaCBL and CaCIPK genes were altered when the plant was exposed to stress. In particular, we found that CaCIPK1 is mediates the pepper plant's defense against P. capsici. These results provide the groundwork for further functional characterization of CaCBL and CaCIPK genes in pepper.