文献类型： 外文期刊

作者： Yang, Shipeng ¹ ; Gao, Jieming ¹ ; Wang, Lihui ¹ ; Sun, Xuemei ¹ ; Xu, Panpan ¹ ; Zhang, Liwen ³ ; Zhong, Qiwen ¹ ;

作者机构： 1.Qinghai Univ, Qinghai Acad Agr & Forestry Sci, Qinghai Key Lab Vegetable Genet & Physiol, Acad Agr & Forestry Sci, Xining 810016, Qinghai, Peoples R China

2.Qinghai Univ, Xining 810016, Qinghai, Peoples R China

3.Shanghai Biochip Co Ltd, Shanghai 201203, Peoples R China

4.Natl Engn Ctr Biochip Shanghai, Shanghai 201203, Peoples R China

关键词： Dormancy; MADS-box transcription factors; Gene expression; Helianthus tuberosus L

期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）

ISSN： 2190-572X

年卷期： 2019 年 9 卷 10 期

页码：

收录情况： SCI

摘要： Dormancy-associated MADS-box (DAM) genes play an important role in plant dormancy and release phases. Little is known about the dormancy characteristics of Jerusalem artichoke tubers. Using bioinformatics, we identified and annotated 23 MADS-box gene sequences from the genome of the Jerusalem artichoke and we analyzed the differential expression of these genes at different developmental stages of tuber dormancy. The results show that all 23 genes encode basic proteins and most of the genes of the same subgroup have similar pI values. MADS-box genes from the Jerusalem artichoke and from other closely related species were divided into ten categories using phylogenetic analysis software. Based on the amino acid sequence of the MADS-domain proteins, ten highly conserved motifs were identified. Gene ontology annotation, InterProScan protein function prediction, and RT-PCR analysis showed that ten MADS-box genes play important roles in the dormancy process of Jerusalem artichoke tubers. Our work lays a foundation for further study of the role of MADS-box genes in the dormancy of the Jerusalem artichoke and other tuber crops.