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Rhamnolipid Enhances the Nitrogen Fixation Activity of Azotobacter chroococcum by Influencing Lysine Succinylation

文献类型： 外文期刊

作者： Li, Jin 1 ; Pan, Hu 2 ; Yang, Hui 1 ; Wang, Chong 1 ; Liu, Huhu 1 ; Zhou, Hui 3 ; Li, Peiwang 4 ; Li, Changzhu 4 ; Lu, Xiang 1 ;

作者机构： 1.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China

2.Tibet Acad Agr & Anim Husb Sci, Inst Agr Prod Qual Stand & Testing Res, Lhasa, Peoples R China

3.Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Peoples R China

4.Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, Changsha, Peoples R China

关键词： nitrogen fixation; Azotobacter chroococcum; posttranslational modifications; lysine succinylation (K-Suc); rhamnolipid

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： The enhancement of nitrogen fixation activity of diazotrophs is essential for safe crop production. Lysine succinylation (K-Suc) is widely present in eukaryotes and prokaryotes and regulates various biological process. However, knowledge of the extent of K-Suc in nitrogen fixation of Azotobacter chroococcum is scarce. In this study, we found that 250 mg/l of rhamnolipid (RL) significantly increased the nitrogen fixation activity of A. chroococcum by 39%, as compared with the control. Real-time quantitative reverse transcription PCR (qRT-PCR) confirmed that RL could remarkably increase the transcript levels of nifA and nifHDK genes. In addition, a global K-Suc of A. chroococcum was profiled using a 4D label-free quantitative proteomic approach. In total, 5,008 K-Suc sites were identified on 1,376 succinylated proteins. Bioinformatics analysis showed that the addition of RL influence on the K-Suc level, and the succinylated proteins were involved in various metabolic processes, particularly enriched in oxidative phosphorylation, tricarboxylic acid cycle (TCA) cycle, and nitrogen metabolism. Meanwhile, multiple succinylation sites on MoFe protein (NifDK) may influence nitrogenase activity. These results would provide an experimental basis for the regulation of biological nitrogen fixation with K-Suc and shed new light on the mechanistic study of nitrogen fixation.

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