Characterization of 17 beta-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater
文献类型： 外文期刊
作者： Hao, Peng 1 ; Pan, Hanyu 1 ; Lv, Zongshuo 1 ; Zhang, Jingyi 1 ; Wang, Lixia 3 ; Zhu, Yanbin 2 ; Basang, Wangdui 2 ; Gao, Yunhang 1 ;
作者机构： 1.Jilin Agr Univ, Coll Vet Med, Changchun 130118, Peoples R China
2.Tibet Acad Agr & Anim Husb Sci, Inst Anim Husb & Vet Med, Lhasa 850009, Peoples R China
3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Changchun 130102, Peoples R China
关键词： 17 beta-estradiol; Enzyme; Remediation; Wastewater; Microbacterium sp. MZT7
期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：6.4； 五年影响因子：6.3 ）
ISSN：
年卷期： 2023 年 22 卷 1 期
页码：
收录情况： SCI
摘要： Background 17 beta-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies having great potential for removal E2 residues from the environment. However, limited literature is available on the removal of E2 from wastewater using short-chain dehydrogenase. Results In this study, 17 beta-estradiol degrading enzyme (17 beta-HSD-0095) was expressed and purified from Microbacterium sp. MZT7. The optimal pH and temperature for reaction was 7 and 40 degrees C, respectively. Molecular docking studies have shown that the ARG215 residue form a hydrogen bond with oxygen atom of the substrate E2. Likewise, the point mutation results have revealed that the ARG215 residue play an important role in the E2 degradation by 17 beta-HSD-0095. In addition, 17 beta-HSD-0095 could remediate E2 contamination in synthetic livestock wastewater. Conclusions These findings offer some fresh perspectives on the molecular process of E2 degradation and the creation of enzyme preparations that can degrade E2.
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