Transcriptomic, Proteomic and Metabolomic Analysis of Flavonoid Biosynthesis During Fruit Maturation in Rubus chingii Hu
文献类型: 外文期刊
作者: Li, Xiaobai 1 ; Jiang, Jingyong 2 ; Chen, Zhen 3 ; Jackson, Aaron 1 ;
作者机构: 1.Zhejiang Acad Agr Sci, Hangzhou, Peoples R China
2.Taizhou Acad Agr Sci, Linhai, Peoples R China
3.Taizhou Univ, Coll Life Sci, Taizhou, Peoples R China
关键词: flavanol-anthocyanins; gene; enzyme; flavonoid biosynthesis; Chinese raspberry
期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:5.754; 五年影响因子:6.612 )
ISSN: 1664-462X
年卷期: 2021 年 12 卷
页码:
收录情况: SCI
摘要: Rubus chingii HU, is a medicinal and nutritious fruit, which is very rich in flavonoids. However, the biosynthesis of its flavonoids is poorly understood. This study examined flavonoids and the genes/proteins at four fruit ripening phases using LC-MS/MS and qPCR. Six major kinds of anthocyanins, primarily consisted of flavanol-anthocyanins, which differed in form or concentration from other Rubus species. In contrast to other known raspberries species, R. chingii had a decline in flavonoids during fruit ripening, which was due to down-regulation of genes and proteins involved in phenylpropanoid and flavonoid biosynthesis. Unexpectedly, anthocyanin also continuously decreased during fruit maturation. This suggests that anthocyanins are not responsible for the fruit's reddish coloration. Flavanol-anthocyanins were derived from the proanthocyanidin pathway, which consumed two flavonoid units both produced through the same upstream pathway. Their presence indicates a reduction in the potential biosynthesis of anthocyanin production. Also, the constantly low expression of RchANS gene resulted in low levels of anthocyanin biosynthesis. The lack of RchF3 ' 5 ' H gene/protein hindered the production of delphinidin glycosides. Flavonoids primarily comprising of quercetin/kaempferol-glycosides were predominately located at fruit epidermal-hair and placentae. The proportion of receptacle/drupelets changes with the maturity of the fruit and may be related to a decrease in the content of flavonoids per unit mass as the fruit matures. The profile and biosynthesis of R. chingii flavonoids are unique to Rubus. The unique flavonol pathways of R. chingii could be used to broaden the genetic diversity of raspberry cultivars and to improve their fruit quality.
- 相关文献
作者其他论文 更多>>
-
Dynamical Regulation of Photosynthetic Components Related to Photosynthesis and Photoprotection in Maturing Blueberry Fruit
作者:Li, Xiaobai;Jin, Liang;Pan, Xuhao;Liu, Li;Wen, Xing;Yu, Fei;Guo, Weidong
关键词:Fruit photosynthesis; Ripening; Pigments; Photoprotection; Proteomics; Vaccinium corymbosum
-
Quantitative trait loci associated with straighthead-resistance used for marker assisted selection in rice (Oryza sativa L.) RIL populations
作者:Pan, Xuhao;Li, Yiting;Pan, Xuhao;Li, Xiaobai
关键词:Straighthead; Resistant breeding; QTLs; Gene effect; Oryza sativa L; Marker-assisted selection
-
Evolution of pathogenicity in obligate fungal pathogens and allied genera
作者:RoyChowdhury, Moytri;Sternhagen, Jake;Xin, Ya;Li, Chunnan;Lou, Binghai;Li, Xiaobai
关键词:Obligate Pathogens; Parasitism; Virulance; Pathogenicity; Biotrophy; Evolution
-
Characterization of carotenoids and phenolics during fruit ripening of Chinese raspberry (Rubus chingii Hu)
作者:Li, Xiaobai;Sun, Jian;Chen, Zhen;Jiang, Jingyong;Jackson, Aaron
关键词:
-
Bioactive compounds and fruit quality of Chinese raspberry, Rubus chingii Hu varied with genotype and phenological phase
作者:Chen, Zhen;Xie, Yiwen;Jin, Zexin;Wang, Xiaoyan;Li, Yueling;Zhong, Yongjun;Lin, Jianjie;Yang, Wanqin;Jiang, Jingyong;Li, Xiaobai
关键词:Rubus chingii Hu; Phenological phase; Fruit quality; Ellagic acid; Kaempferol-3-O-rutinoside
-
Combined transcriptomic and metabolic analyses reveal potential mechanism for fruit development and quality control of Chinese raspberry (Rubus chingii Hu)
作者:Chen, Zhen;Shu, Liangzuo;Huang, Jing;Qian, Baoying;Wang, Xiaoyan;Li, Xin;Chen, Jiangxia;Jiang, Jingyong;Li, Xiaobai;Xu, Haidan
关键词:Rubus chingii Hu; Transcriptome; Metabolome; Ellagic acid; Kaempferol-3-O-rutinoside
-
Dynamic regulation of small RNAs in anthocyanin accumulation during blueberry fruit maturation
作者:Li, Xiaobai;Guo, Fangqi;Hong, Yan
关键词: