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Molecular detection of Enterocytozoon bieneusi in farm-raised pigs in Hainan Province, China: infection rates, genotype distributions, and zoonotic potential

文献类型: 外文期刊

作者: Zhou, Huan-Huan 1 , 2 , 3 ; Ma, Tian-Ming 1 , 2 , 3 ; Zhou, Jing-Guo 1 , 2 , 3 ; Liu, Hai-Ju 1 , 2 , 3 ; Lu, Gang 1 , 2 , 3 ; Zhao, Wei 1 , 2 , 3 , 6 ; Zheng, Xin-Li 2 ; Qi, Meng 2 ;

作者机构: 1.Hainan Med Univ, Dept Pathogen Biol, Haikou 571199, Hainan, Peoples R China

2.Hainan Med Univ, Hainan Med Univ Univ Hong Kong Joint Lab Trop Inf, Haikou 571199, Hainan, Peoples R China

3.Hainan Med Univ, Minist Educ, Key Lab Trop Translat Med, Haikou 571199, Hainan, Peoples R China

4.Tarim Univ, Coll Anim Sci, Alar 843300, Xinjiang, Peoples R China

5.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Hainan, Peoples R China

6.Wenzhou Med Univ, Dept Parasitol, Wenzhou 325035, Zhejiang, Peoples R China

关键词: Enterocytozoon bieneusi; Genotype; ITS region; Pigs

期刊名称:PARASITE ( 影响因子:2.05; 五年影响因子:2.49 )

ISSN: 1252-607X

年卷期: 2020 年 27 卷

页码:

收录情况: SCI

摘要: Enterocytozoon bieneusi is a zoonotic fungal pathogen with a high degree of host diversity that can parasitize many animals, including humans. Pigs may play an important role in the epidemiology of E. bieneusi as reservoir hosts. Nevertheless, the genotypes of E. bieneusi in pigs in China remain poorly understood. The aim of this study was to determine the prevalence of E. bieneusi infection amongst pigs raised on farms from four cities of Hainan Province, using nested polymerase chain reaction (PCR) of the partial small subunit of the ribosomal RNA gene, and to identify genotypes of E. bieneusi isolates based on sequence analysis of the ribosomal internal transcribed spacer (ITS) region. Among 188 stool samples, E. bieneusi was detected in 46.8% (88/188). Eight genotypes including four known (EbpA, CS-4, MJ14, and CHG19) and four novel (HNP-I - HNP-IV) genotypes were identified. Using phylogenetic analysis, genotypes EbpA, CS4, CHG19, HNP-III, and HNP-IV were clustered into zoonotic Group 1, while the remaining three genotypes (MJ14, HNP-I, and HNP-II) clustered into Group 10. The high prevalence of zoonotic genotypes of E. bieneusi among pigs suggests that pig farming is a potential source of human infection. Additionally, this is the first identification of genotypes in Group 10 in pigs indicating unique epidemic features of E. bieneusi in pigs in Hainan Province, the southernmost part of China.

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