Truncated affinity-improved aptamers for 17 beta-estradiol determination by AuNPs-based colorimetric aptasensor
文献类型： 外文期刊
作者： Qiao, Lu 1 ; Wang, He 3 ; He, Junlin 2 ; Yang, Shuming 1 ; Chen, Ailiang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Zhongguancun South St 12, Beijing 100081, Peoples R China
2.Acad Mil Med Sci, Beijing Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Taiping Rd 27, Beijing 100850, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Agr Stand & Testing, 9 Middle Rd Shuguanghuayuan, Beijing 100097, Peoples R China
关键词： 17 beta-estradiol; Truncated aptamers; Binding domain; AuNPs; Colorimetric aptasensor
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2021 年 340 卷
页码：
收录情况： SCI
摘要： 17 beta-estradiol (E2) residues could enrich in organisms via food chain and lead to harmful biological effects for human body. To ascertain the binding domain of original E2 aptamer (E00) with long-sequence (76-mer), we developed novel truncated aptamers from E00, through rationally designed truncation by intercepting a single ring or a combination of rings (containing hairpin loop, interior loop or multiloop) at different sites and retaining appropriate double helix regions. Through comparison, 15-mer E09 presented improved affinity and higher specificity, indicating the hairpin loop near to 3 ' end of E00 served on the binding domain to E2. E09 was used for gold nanoparticles (AuNPs)-based colorimetric determination of E2, achieved the detection limit of 0.02 mu g/mL. The truncated aptamer (only 15-mer) first proposed in this study has great application potential in E2 determination, and this work provides proof-of-concept study for truncation of other long-sequence aptamers.
- 相关文献
作者其他论文 更多>>
-
Emergence of Antifungal Resistant Subclades in the Global Predominant Phylogenetic Population of Candida albicans
作者：Gong, Jie;Xu, Juan;Zhang, Shu;Geng, Yuan-Yuan;Zhao, Fei;Lu, Xin;He, Li-Hua;Zhang, Jian-Zhong;Chen, Xin-Fei;Zhang, Han;Xu, Ying-Chun;Xiao, Meng;Chen, Xin-Fei;Zhang, Han;Xu, Ying-Chun;Xiao, Meng;Fan, Xin;Fan, Xin;Li, Ruo-Yu;Wan, Zhe;Li, Ruo-Yu;Wan, Zhe;Li, Ruo-Yu;Chen, Sharon C-A;Kong, Fanrong;Sun, Zi-Yong;Kang, Mei;Liao, Kang;Guo, Da-Wen;Hu, Zhi-Dong;Chu, Yun-Zhuo;Zhao, Hong-Mei;Zou, Gui-Ling;Shen, Chong;Shen, Chong;Wu, Wei-Wei;Wang, He;Liu, Gui-Ming
关键词：Candida albicans; phylogenetic population; azole-resistance; Erg11p; Tac1p; azole-nonsusceptibility
-
Shortened and multivalent aptamers for ultrasensitive and rapid detection of alternariol in wheat using optical waveguide sensors
作者：Wang, Shuo;Gao, Hualong;Wei, Zhenzhe;Zhou, Jianshuo;Ren, Shang;Lou, Xinhui;He, Junlin;Luan, Yunxia
关键词：Aptamer; Multivalent; Critical binding site; Alternariol; Aptasensor; Waveguide sensor
-
A Lateral Flow Strip Based on a Truncated Aptamer-Complementary Strand for Detection of Type-B Aflatoxins in Nuts and Dried Figs
作者：Zhao, Zhilei;Wang, He;Wang, Meng;Zhao, Zhilei;Wang, He;Zhai, Wenlei;Feng, Xiaoyuan;Wang, Meng;Fan, Xia;Chen, Ailiang
关键词：Type-B aflatoxins; lateral flow; aptamer; complementary strand; nuts and dried figs
-
Identification of odorant-binding proteins and functional analysis of antenna-specific AplaOBP1 in the emerald ash borer, Agrilus planipennis
作者：Wang, Shan-Ning;Yu, Guo-Yue;Zhang, Fan;Shan, Shuang;Khashaveh, Adel;Zhang, Yong-Jun;Wang, He;Shan, Shuang;Dhiloo, Khalid Hussain
关键词：Agrilus planipennis; Odorant-binding proteins; Sensilla basiconica; Fluorescence binding assay; Electroantennography
-
Correlation between population size of pear Psylla (Cacopsylla chinensis) and leaf structure features in different pear cultivars
作者：Xu, Sheng-jie;Wu, Lun;Liu, Qi-zhi;Wang, Jie-wen;Zhang, Ting;Liu, Jun;Wang, He
关键词：Pear breeding; Cacopsylla chinensis; Antixenosis; Leaf tissue; Resistance
-
Genetic traceability practices in a large-size beef company in China
作者：Zhao, Jie;Xu, Zhenzhen;You, Xinyong;Zhao, Yan;He, Wenjing;Zhao, Luyao;Chen, Ailiang;Yang, Shuming;Zhao, Jie;Xu, Zhenzhen;You, Xinyong;Zhao, Yan;He, Wenjing;Zhao, Luyao;Chen, Ailiang;Yang, Shuming;Zhao, Jie;You, Xinyong
关键词：Genetic traceability practice; SNP markers; Meat traceability; Chinese enterprise
-
Characterization and identification of cold tolerant near-isogenic lines in rice
作者：Zhou, Lei;Hu, Guanglong;Pan, Yinghua;Zhang, Hongliang;Li, Jinjie;Li, Zichao;Zhou, Lei;Hu, Guanglong;Pan, Yinghua;Zhang, Hongliang;Li, Jinjie;Li, Zichao;Zeng, Yawen;Yang, Shuming;Zhou, Lei;You, Aiqing;Hu, Guanglong
关键词：Oryza sativa;cold tolerance;booting stage;near-isogenic lines
