A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination
文献类型: 外文期刊
作者: Li, Shuangtao 1 ; Wang, Zhirong 1 ; Wang, Fei 1 ; Lv, Hongmei 1 ; Cao, Meng 1 ; Zhang, Na 1 ; Li, Fengju 3 ; Wang, Hao 3 ; L 1 ;
作者机构: 1.China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China
3.Tianjin Acad Agr Sci, Tianjin 300192, Peoples R China
4.Shandong Huasheng Agr Co Ltd, Shandong Prov Key Lab Cucurbitaceae Vegetable Bio, Qingzhou 262500, Shandong, Peoples R China
关键词: Cucumis sativus L; E3 ubiquitin ligase; Salt stress; Osmotic stress; Transcription factor; Tubby-like protein
期刊名称:BMC PLANT BIOLOGY ( 影响因子:4.215; 五年影响因子:4.96 )
ISSN: 1471-2229
年卷期: 2021 年 21 卷 1 期
页码:
收录情况: SCI
摘要: Background TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. Results In cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure. Conclusion Under osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.
- 相关文献
作者其他论文 更多>>
-
Integrative genomic and transcriptomic analyses of a bud sport mutant ?Jinzao Wuhe? with the phenotype of large berries in grapevines
作者:Huang, Jianquan;Li, Yanhao;Zhang, He;Zhang, Na;Zhang, Guan;Lyu, Mingjie;Chen, Rui;Zhang, Guan;Li, Yanhao
关键词:Grapevine; Bud sport; Berry enlargement; Genetic variation; RNA-seq; WGRS
-
Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis
作者:Zhao, Yuehan;Guo, Honglian;Zhao, Yuehan;Zhang, Na;Ji, Haipeng;Dong, Chenghu;Yu, Jinze;Chen, Cunkun;Hou, Zhaohua;Ji, Haipeng;Chen, Xueling
关键词:sweet cherry (Prunus avium L; ); ozone; label-free quantification proteomics; preservation; mfuzz
-
Effect of Cold Plasma Treatment on the Softening of Winter Jujubes (Ziziphus jujuba Mill. cv. Dongzao)
作者:Jia, Sitong;Yan, Shijie;Zhang, Na;Dong, Chenghu;Zheng, Pufan;Ji, Haipeng;Yu, Jinze;Chen, Cunkun;Liang, Liya
关键词:cold plasma; winter jujubes (Ziziphus jujuba Mill. cv. Dongzao); softening; storage; cell wall-degrading enzymes
-
Stability of exogenous Cadmium in different vineyard soils and its effect on grape seedlings
作者:Mamut, Ajigul;Zhou, Hu;Lv, Yizhong;Mamut, Ajigul;Andom, Okbagaber;Li, Zhaojun;Huang, Jianquan;Zhang, He;Zhang, Na
关键词:Vineyard soil; Cd fractions; Soil type; Community Bureau of Reference (BCR); Inhibition rate
-
Effect of Storage Conditions and Cooking Methods on Chlorophyll, Glucosinolate, and Sulforaphane Content in Broccoli Florets
作者:Wang, Junwei;Mao, Shuxiang;Yuan, Yiming;Wu, Qi;Liang, Mantian;Wang, Shengze;Huang, Ke;Wu, Qiuyun;Wang, Junwei;Mao, Shuxiang;Yuan, Yiming;Wu, Qi;Liang, Mantian;Wang, Shengze;Huang, Ke;Wu, Qiuyun;Wang, Junwei;Mao, Shuxiang;Yuan, Yiming;Wu, Qi;Liang, Mantian;Wang, Shengze;Huang, Ke;Wu, Qiuyun;Zhang, Na
关键词:broccoli; storage; cooking; glucosinolate; sulforaphane
-
A new species of gall midge (Diptera: Cecidomyiidae) damaging flower buds of goji berry Lycium barbarum (Solanaceae)
作者:Jiao, Ke-Long;Zhou, Xiao-Yi;Wang, Hao;Qiao, Hai-Li;Chen, Jun;Liu, Bai-Ming;Bu, Wen-Jun;Kolesik, Peter
关键词:Goji gall midge; Lycium barbarum; Lycium ruthenicum; Insect pest
-
Crystal Structure of African Swine Fever Virus pS273R Protease and Implications for Inhibitor Design
作者:Li, Guobang;Liu, Xiaoxia;Yang, Mengyuan;Zhang, Guangshun;Wang, Zhengyang;Guo, Kun;Gao, Yuxue;Jiao, Peng;Sun, Jixue;Wang, Hao;Deng, Weilong;Xiao, Huihe;Li, Sizheng;Wu, Haoru;Cao, Lin;Shang, Luqing;Yang, Cheng;Guo, Yu;Rao, Zihe;Li, Guobang;Liu, Xiaoxia;Yang, Mengyuan;Zhang, Guangshun;Wang, Zhengyang;Guo, Kun;Gao, Yuxue;Jiao, Peng;Sun, Jixue;Wang, Hao;Deng, Weilong;Xiao, Huihe;Li, Sizheng;Wu, Haoru;Cao, Lin;Shang, Luqing;Yang, Cheng;Guo, Yu;Rao, Zihe;Li, Guobang;Liu, Xiaoxia;Yang, Mengyuan;Zhang, Guangshun;Wang, Zhengyang;Guo, Kun;Gao, Yuxue;Jiao, Peng;Wang, Hao;Deng, Weilong;Wu, Haoru;Cao, Lin;Shang, Luqing;Yang, Cheng;Guo, Yu;Rao, Zihe;Liu, Xiaoxia;Wang, Zhengyang;Guo, Kun;Jiao, Peng;Yang, Cheng;Guo, Yu;Rao, Zihe;Cao, Lin;Jia, Zihan;Rao, Zihe;Chen, Cheng;Wang, Ying;Jia, Zihan;Guo, Yu;Rao, Zihe
关键词:ASFV; protease; crystal structure; inhibitor design