Adaptive responses and metabolic strategies of Novosphingobium sp. ES2-1-17β-estradiol analyzed through integration of genomic and proteomic approaches
文献类型: 外文期刊
作者: Li, Shunyao 1 ; Yang, Wei 1 ; Mo, Jingjing 1 ; Wang, Yubing 1 ; Lu, Chao 4 ; Gao, Yanzheng 2 ; Li, Yucheng 1 ; Sun, Kai 3 ;
作者机构: 1.Anhui Univ, Anhui Prov Key Lab Wetland Ecosyst Protect & Resto, Jiulong Rd 111, Hefei 230601, Peoples R China
2.Nanjing Agr Univ, Inst Organ Contaminant Control & Soil Remediat, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China
3.Anhui Agr Univ, Coll Resources & Environm, Anhui Prov Key Lab Farmland Ecol Conservat & Pollu, Hefei 230036, Anhui, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Natl Agr Expt Stn Agr Environm, Nanjing 210014, Peoples R China
关键词: Microbial degradation; Steroid estrogens; Novosphingobium; Molecular mechanisms; Genomics and proteomics
期刊名称:JOURNAL OF HAZARDOUS MATERIALS ( 影响因子:13.6; 五年影响因子:12.7 )
ISSN: 0304-3894
年卷期: 2024 年 461 卷
页码:
收录情况: SCI
摘要: Environmental 17 beta-estradiol (E2) can cause potential harm to ecological balance and human health. Novosphingobium sp. ES2-1 is an E2-degrading bacterium previously obtained, which converts E2 to estrone (E1) and then to 4-hydroxyestrone (4-OH-E1) followed by oxidation to form metabolites with long-chain structure during upstream degradation. Herein, we found that intracellular enzymes were the major contributors to E2 biodegradation by strain ES2-1. A total of 243 proteins were dys-expressed under E2 condition, 123 were up-regulated and 120 were down-regulated thereinto. The up-regulated members of ABC transport systems, aromatics degradation, and fatty acid degradation indicated a reinforced transfer and utilization of E2. Cytochrome P450 monooxygenase (EstP1), 2-keto-4-pentenoate hydratase, pyruvate dehydrogenase, acetyl-CoA acetyltransferase, TonB-dependent receptor were involved in E2 catabolism. During downstream degradation, the metabolites with long-chain structure were decomposed adopting beta-oxidation pattern and ultimately entered the TCA cycle; 2 keto-4-pentenoic acid might be an emblematic product of such process. Furthermore, E2 converting to E1 was catalyzed by 17 beta-dehydrogenase probably encoded by IM701_16645 or IM701_16910; 4-OH-E1 meta-cleavage was catalyzed by a dioxygenase encoded by IM701_20340 or IM701_21000 or IM701_09625. Our study provided an in-depth insight into the adaptive responses and metabolic strategies of Novosphingobium to E2.
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