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Plant volatile ligands for male-biased MmedOBP14 stimulate orientation behavior of the parasitoid wasp Microplitis mediator

文献类型： 外文期刊

作者： Li, Ruijun 1 ; Shan, Shuang 2 ; Song, Xuan 1 ; Khashaveh, Adel 2 ; Wang, Shanning 3 ; Yin, Zixuan 2 ; Lu, Ziyun 4 ; Dhiloo, Khalid Hussain 5 ; Zhang, Yongjun 2 ;

作者机构： 1.Hebei Agr Univ, Coll Plant Protect, Baoding 071000, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing Key Lab Environm Friendly Management Fruit, Beijing 100097, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, IPM Ctr Hebei Prov, Key Lab Integrated Pest Management Crops Northern, Baoding 071000, Hebei, Peoples R China

5.Sindh Agr Univ, Fac Crop Protect, Dept Entomol, Tandojam 70060, Pakistan

关键词： Odorant binding protein; Microplitis mediator; Electrophysiological and behavioral responses

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 223 卷

页码：

收录情况： SCI

摘要： As an important class of chemosensory-associated proteins, odorant binding proteins (OBPs) play a key role in the perception of olfactory signals for insects. Parasitoid wasp Microplitis mediator relies on its sensitive olfactory system to locate host larvae of Noctuidae and Geometridae. In the present study, MmedOBP14, a male-biased OBP in M. mediator, was functionally investigated. In fluorescence competitive binding assays, the recombinant MmedOBP14 showed strong binding abilities to five plant volatiles: beta-ionone, 3,4-dimethylacetophenone, 4ethylacetophenone, acetophenone and ocimene. Homology modeling and molecular docking results indicated that the binding sites of all five ligands were similar and concentrated in the binding pocket of MmedOBP14. Except acetophenone, the remaining four ligands at 1, 10 and 100 mu g/mu L caused strong antennal electrophysiological responses in adults M. mediator, and males showed more obvious EAG responses to most ligands than females. In behavioral trials, males were attracted by low concentrations of MmedOBP14 ligands, whereas high doses of beta-ionone and acetophenone had a repellent effect on males. Moreover, 1 mu g/mu L of 3,4-dimethylacetophenone showed the strongest attractiveness to female wasps. These findings suggest that MmedOBP14 may play a more important role in the perception of plant volatiles for male wasps to locate habitat, supplement nutrition and search partners.

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