Integrated metabolomics and lipidomics analyses suggest the temperature-dependent lipid desaturation promotes aflatoxin biosynthesis in Aspergillus flavus
文献类型: 外文期刊
作者: Wu, Shaowen 1 ; Huang, Wenjie 1 ; Wang, Fenghua 1 ; Zou, Xinlu 1 ; Li, Xuan 1 ; Liu, Chun-Ming 2 ; Zhang, Wenyang 1 ; Yan, Shijuan 1 ;
作者机构: 1.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
关键词: Aspergillus flavus; aflatoxins; metabolomics; lipidomics; temperature
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.2; 五年影响因子:6.2 )
ISSN:
年卷期: 2023 年 14 卷
页码:
收录情况: SCI
摘要: Temperature is one of the main factors affecting aflatoxin (AF) biosynthesis in Aspergillus flavus. Previous studies showed that AF biosynthesis is elevated in A. flavus at temperatures between 28 degrees C-30 degrees C, while it is inhibited at temperatures above 30 degrees C. However, little is known about the metabolic mechanism underlying temperature-regulated AF biosynthesis. In this study, we integrated metabolomic and lipidomic analyses to investigate the endogenous metabolism of A. flavus across 6 days of mycelia growth at 28 degrees C (optimal AF production) and 37 degrees C (no AF production). Results showed that both metabolite and lipid profiles were significantly altered at different temperatures. In particular, metabolites involved in carbohydrate and amino acid metabolism were up-regulated at 37 degrees C on the second day but down-regulated from days three to six. Moreover, lipidomics and targeted fatty acids analyses of mycelia samples revealed a distinct pattern of lipid species and free fatty acids desaturation. High degrees of polyunsaturation of most lipid species at 28 degrees C were positively correlated with AF production. These results provide new insights into the underlying metabolic changes in A. flavus under temperature stress.
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