Complete biodegradation of the oldest organic herbicide 2,4-Dichlorophe- noxyacetic acid by engineering Escherichia coli
文献类型： 外文期刊
作者： Wang, Yu 1 ; Tian, Yong-Sheng 1 ; Gao, Jian-Jie 1 ; Xu, Jing 1 ; Li, Zhen-Jun 1 ; Fu, Xiao-Yan 1 ; Han, Hong-Juan 1 ; Wang, Li-Juan 1 ; Zhang, Wen-Hui 1 ; Deng, Yong-Dong 1 ; Qian, Cen 1 ; Zuo, Zhi-Hao 1 ; Wang, Bo 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
关键词： 24-Dichlorophenoxyacetic acid; Biodegradation; Environmental pollution; Engineered bacteria; Synthetic biology
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）
ISSN： 0304-3894
年卷期： 2023 年 451 卷
页码：
收录情况： SCI
摘要： After nearly 80 years of extensive application, the oldest organic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) has caused many problems of environmental pollution and ecological deterioration. Bioremediation is an ideal method for pollutant treatment. However, difficult screening and preparation of efficient degradation bacteria have largely hindered its application in 2,4-D remediation. We have created a novel engineering Escherichia coli with a reconstructed complete degradation pathway of 2,4-D to solve the problem of screening highly efficient degradation bacteria in this study. The results of fluorescence quantitative PCR demonstrated that all nine genes in the degradation pathway were successfully expressed in the engineered strain. The engineered strains can quickly and completely degrade 0.5 mM 2, 4-D within 6 h. Inspiring, the engineered strains grew with 2,4-D as the sole carbon source. By using the isotope tracing method, the metabolites of 2,4-D were found incorporated into the tricarboxylic acid cycle in the engineering strain. Scanning electron microscopy showed that 2,4-D had less damage on the engineered bacteria than the wild-type strain. Engineered strain can also rapidly and completely remedy 2,4-D pollution in natural water and soil. Assembling the metabolic pathways of pollutants through synthetic biology was an effective method to create pollutant-degrading bacteria for bioremediation.
- 相关文献
作者其他论文 更多>>
-
A dual-RPA based lateral flow strip for sensitive, on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops
作者：Wang, Jinbin;Wang, Yu;Hu, Xiuwen;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Jinbin;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Yu;Liu, Xiaofeng;Liu, Juan;Zhu, Lemei;Liu, Juan;Zhu, Lemei;Zeng, Haijuan;Liu, Hua
关键词：Genetically modified crops; On-site detection; Lateral flow test strips; Dual recombinase polymerase amplification; (RPA)
-
Metabolic engineering for the biosynthesis of bis-indolylquinone terrequinone A in Escherichia coli from L-tryptophan and prenol
作者：Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Gao, Jianjie;Li, Zhenjun;Zhang, Wenhui;Xu, Jing;Wang, Bo;Wang, Yu;Han, Hongjuan;Fu, Xiaoyan;Tian, Yongsheng;Yao, Quanhong;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Gao, Jianjie;Li, Zhenjun;Zhang, Wenhui;Xu, Jing;Wang, Bo;Wang, Yu;Han, Hongjuan;Fu, Xiaoyan;Tian, Yongsheng;Yao, Quanhong
关键词：Biosynthesis; Bis-indolylquinone; Terrequinone A; Dimethylallyl diphosphate (DMAPP); L-tryptophan (L-Trp); Escherichia coli
-
Response regulator KdpE contributes to Aeromonas dhakensis virulence
作者：Chen, Liqing;Wang, Yu;Fan, Lixia;Yang, Nuo;Zeng, Jifeng;Guo, Guiying;Li, Qian;Zheng, Jiping;Zeng, Jifeng;Zheng, Jiping;Wang, Yu;Wang, Peng;Zeng, Weixin
关键词：Aeromonas dhakensis; KdpE; Attenuated virulence; Histopathology; Zebrafish
-
Genetic engineering of complex feed enzymes into barley seed for direct utilization in animal feedstuff
作者：Peng, Ri-He;Zhang, Wen-Hui;Wang, Yu;Deng, Yong-Dong;Wang, Bo;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Fu, Xiao-Yan;Xu, Jing;Han, Hong-Juan;Tian, Yong-Sheng;Yao, Quan-Hong;Peng, Ri-He;Zhang, Wen-Hui;Wang, Yu;Deng, Yong-Dong;Wang, Bo;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Fu, Xiao-Yan;Xu, Jing;Han, Hong-Juan;Tian, Yong-Sheng;Yao, Quan-Hong
关键词：recombinant enzymes; feed additive; feedstuff; nutrient utilization
-
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
作者：Wang, Bo;Wang, Ya-Hui;Deng, Yuan-Jie;Xiong, Ai-Sheng;Wang, Bo;Yao, Quan-Hong
关键词：Genetically modified carrot; Betanin; Unintended effects; Photosynthesis; Transcriptome; Metabolomics
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
