Knockdown of Sly-miR160a using short tandem target mimic (STTM) enhanced expression of auxin signaling genes and delayed postharvest ripening of tomato fruit
文献类型： 外文期刊
作者： Mekontso, Francine Ngaffo 1 ; Wu, Shuhui 1 ; Fu, Ruizuo 1 ; Li, Wen 2 ; Meng, Lanhuan 1 ; Wang, Qing 3 ; Li, Jiangkuo 4 ; Song, Hongmiao 1 ; Xu, Xiangbin 1 ;
作者机构： 1.Hainan Univ, Sch Food Sci & Engn, Haikou 570228, Peoples R China
2.Hainan Univ, Sch Hort, Haikou 570228, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr IAPN, Key Lab Vegetable Postharvest Treatment, Minist Agr,Beijing Key Lab Fruits & Vegetable Sto, Beijing 100097, Peoples R China
4.Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin Key Lab Postharvest Physiol & Storage Agr, Tianjin 300384, Peoples R China
5.Key Lab Food Nutr & Funct Food Hainan Prov, Haikou 570228, Peoples R China
关键词： MiR160a; ARFs; Tomato; Ripening; Auxin signaling
期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：7.0； 五年影响因子：6.9 ）
ISSN： 0925-5214
年卷期： 2023 年 198 卷
页码：
收录情况： SCI
摘要： The miR160 is a highly conserved microRNA (miRNA) that regulates the auxin signaling pathway through negative regulation of auxin response factors (ARFs) and plays pivotal roles in fruit development. In the present study, the knockdown of miR160a using short tandem target mimic (STTM 160a) and overexpression of miR160a (miR160a-OE) transgenic tomato lines were generated to investigate the possible roles of miR160 in postharvest ripening tomato fruit. The results showed that the STTM 160a increased total chlorophyll content, reduced lycopene and total carotenoid accumulation, weight loss, and respiration rate while increasing firmness in to-mato fruit compared with the wild-type (WT) and miR160a-OE. Additionally, miR160a-OE lines exhibited a typical auxin hypersensitivity and postharvest ripening acceleration, whereas STTM 160a lines displayed an auxin hyposensitivity and postharvest ripening reduction. Moreover, the relative expression levels of ARF10A (Solyc11g069500.2.1), ARF10B (Solyc06g075150.3.1), ARF17 (Solyc11g013480.2.1), and Aux/IAA genes were up-regulated in STTM 160a lines. The results suggest that the knockdown of Sly-miR160a delays postharvest ripening in tomato fruit, and provide valuable information for genetic engineering in controlling developmental processes.
- 相关文献
作者其他论文 更多>>
-
Photocatalytic titanium dioxide reduces postharvest decay of nectarine fruit packaged in different materials through modulating central carbon and energy metabolisms
作者：Zheng, Yanli;Duan, Lihua;Li, Xihong;Li, Jiangkuo;Zhang, Peng;Jia, Xiaoyu;Jiang, Yunbin;Jia, Xiaoyu;Yang, Xiangzheng;Yang, Xiangzheng;Jia, Xiaoyu
关键词：TiO 2 photocatalysis; Microperforated LDPE packaging; Postharvest decay; Central carbon metabolism; Energy metabolism
-
Overexpression of Sly-miR164a in tomato decreases expression of NAC and delays pre- and postharvest ripening of fruit
作者：Wang, Zhiqiang;Duan, Wenhui;Meng, Lanhuan;Song, Hongmiao;Xu, Xiangbin;Wang, Qing;Li, Jiangkuo;Xu, Xiangbin
关键词：miR164; Tomato; Ripening; Postharvest; NAC
-
Overexpression of Sly-miR167a delayed postharvest chilling injury of tomato fruit under low temperature storage
作者：Li, Lei;Wang, Xuanqi;Lv, Jieyu;Duan, Wenhui;Huang, Taishan;Zhao, Keyan;Meng, Lanhuan;Song, Hongmiao;Xu, Xiangbin;Zhao, Keyan;Li, Jiangkuo;Zhao, Keyan;Wang, Qing;Xu, Xiangbin;Li, Jiangkuo
关键词：MicroRNA167a; ARF6a; Chilling injury; Tomato fruit; Ethylene
-
Carvacrol exhibits direct antifungal activity against stem-end rot disease and induces disease resistance to stem-end rot disease in kiwifruit
作者：Mi, Tingting;Luo, Donglan;Qu, Guangfan;Sun, Yanzheng;Cao, Sen;Li, Jiangkuo
关键词：Kiwifruit; Carvacrol; Stem-end rot; Antifungal activity; Disease resistance
-
Facile microfluidic fabrication and characterization of ethyl cellulose/PVP films with neatly arranged fibers
作者：Rao, Jingshan;Yang, Zhichao;Wu, Di;Chen, Kunsong;Shen, Chaoyi;Fawole, Olaniyi Amos;Li, Jiangkuo;Wu, Di
关键词：Microfluidic spinning; Neatly arranged fibers; Ethyl cellulose; Polyvinylpyrrolidone; Microfiber films
-
Comparative proteomic analysis of wild-type and a SlETR-3 (Nr) mutant reveals an ethylene-induced physiological regulatory network in fresh-cut tomatoes
作者：Shi, Jun-yan;Wang, Qian;Zuo, Jin-hua;Zheng, Shu-fang;Gao, Li-pu;Liu, Jia;Wang, Qing;Shi, Jun-yan;Wang, Qian;Zuo, Jin-hua;Zheng, Shu-fang;Gao, Li-pu;Wang, Qing;Wang, Qian;Liu, Jia;Shi, Jun-yan
关键词：Fresh-cut tomato; Proteomics; Ethylene; Pectinesterase; Physiological regulatory network
-
Biodegradable gelatin/pullulan aerogel modified by a green strategy: Characterization and antimicrobial activity
作者：Yang, Zhichao;Shen, Chaoyi;Li, Jiawen;Zhang, Hui;Wu, Di;Yang, Zhichao;Shen, Chaoyi;Rao, Jingshan;Yang, Xiangzheng;Wu, Di;Chen, Kunsong;Yang, Zhichao;Shen, Chaoyi;Rao, Jingshan;Wu, Di;Chen, Kunsong;Yang, Zhichao;Shen, Chaoyi;Rao, Jingshan;Wu, Di;Chen, Kunsong;Yang, Xiangzheng;Li, Jiangkuo;Fawole, Olaniyi Amos;Wu, Di
关键词：Gelatin; Pullulan; Maillard reaction; Nisin; Antimicrobial; Biodegradable
