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A Comparative Study on Polyphenolic Composition of Berries from the Tibetan Plateau by UPLC-Q-Orbitrap MS System

文献类型: 外文期刊

作者: Jia, Qiangqiang 1 ; Zhang, Shoude 2 ; Zhang, Hongyang 1 ; Yang, Xijuan 3 ; Cui, Xinli 2 ; Su, Zhanhai 4 ; Hu, Ping 1 ;

作者机构: 1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China

3.Qinghai Acad Agr & Forestry Sci, Tibetan Plateau Key Lab Agr Prod Proc, Xining 810016, Peoples R China

4.Qinghai Univ, Dept Pharm, Med Coll, Xining 810001, Peoples R China

关键词: berries; Tibetan plateau; orbitrap MS; phenolic compounds

期刊名称:CHEMISTRY & BIODIVERSITY ( 影响因子:2.408; 五年影响因子:2.351 )

ISSN: 1612-1872

年卷期: 2020 年 17 卷 4 期

页码:

收录情况: SCI

摘要: Five traditional medicinal food from the Tibetan plateau including Nitraria tangutorum Bobrov (NT), Hippophae rhamnoides L. (HR), Lycium ruthenicum Murray (LR), Lycium barbarum L. (LB) and Rubus corchorifolius L.f. (RC) are rich in phenolic compounds. However, the detailed studies about the phenolic compounds remain scarce. Therefore, we established a rapid method for the simultaneous identification and quantification of the phenolic compounds from berries via Ultra Performance Liquid Chromatography-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS). This method was verified from many aspects including detection limit, quantification limit, precision, repeatability, stability, average recovery rate and recovery range, and then was used to analyze the phenolic compounds in these five species of berries. Finally, a total of 21 phenolic compounds were directly identified by comparing the retention time and exact mass, of which 14 compounds were identified by us for the first time in berries from the Tibetan plateau, including one flavonoid aglycone (myricetin), 11 phenolic acids (gallic acid, protocatechuate, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, 2-hydroxybenzeneacetic acid and ellagic acid), one flavanol (catechin) and one dihydrochalcone flavonoid (phloretin). Quantitative results showed that rutin, myricetin, quercetin and kaempferol were the main flavonoids. Moreover, a variety of phenolic acid compounds were also detected in most of the berries from the Tibetan plateau. Among these compounds, the contents of protocatechuate and chlorogenic acid were high, and high levels of catechin and phloretin were also detected in these plateau berries.

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