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Integrated metabolomic and transcriptomic analysis of specialized metabolites and isoflavonoid biosynthesis in Sophora alopecuroides L. under different degrees of drought stress

文献类型： 外文期刊

作者： Huang, Xiang 1 ; Chu, Guangming 1 ; Wang, Jun 2 ; Luo, Honghai 1 ; Yang, Zhenan 3 ; Sun, Lupeng 1 ; Rong, Wenwen 1 ; Wang, Mei 1 ;

作者机构： 1.Shihezi Univ, Coll Agr, Shihezi 832003, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Shihezi 832003, Xinjiang, Peoples R China

3.Shihezi Univ, Coll Sci, Shihezi 832003, Xinjiang, Peoples R China

4.Shihezi Univ, Coll Agr, Beisi Rd, Shihezi 832003, Xinjiang, Peoples R China

关键词： Sophora alopecuroides L; Specialized metabolites; Isoflavonoid; Transcriptome; Metabolome; Drought stress

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）

ISSN： 0926-6690

年卷期： 2023 年 197 卷

页码：

收录情况： SCI

摘要： Appropriate drought stress could promote the accumulation of specialized metabolites in plants, especially in medicinal plants. Sophora alopecuroides L. (Leguminosae), a medicinal plant with a variety of bioactive specialized metabolites, has an excellent drought resistance. In this study, drought stress-induced metabolomic and transcriptomic changes in S. alopecuroides were identified, to explore the mechanism of specialized metabolite changes in response to mild, moderate and severe drought stress. The metabolic analysis results showed that a total of 919 annotated metabolites were detected and quantified, of them, flavonoid metabolites were the main metabolites in S. alopecuroides. Moreover, a total of 411 differentially accumulated metabolites (DAMs) were identified, among them, 6-Hydroxydaidzein, 2-Hydroxy-2,3-dihydrogenistein, prunetin, and bio-chanin A significantly increased under mild drought stress but isoflavonoids significantly decreased under moderate and severe drought stress. Transcriptional analysis showed that a total of 21,307 differentially expressed genes (DEGs) were identified, and most of them were enriched in the biosynthesis of secondary me-tabolites pathway. The integrated metabolomic and transcriptomic analyses results proved that these DAMs and DEGs were most significantly enriched in isoflavonoid biosynthesis pathway (ko00943). In addition, some structural genes such as 2-hydroxyisoflavanone synthase (IFS), 2-hydroxyisoflavanone dehydratase (HID), iso-flavone 7-O-glucosyltransferase (IF7GT), and isoflavone 7-O-glucoside-6"-O-malonyltransferase (IF7MAT) and seven MYB transcription factors (SaMYB1 similar to SaMYB7) were identified, which played crucial regulatory roles in the isoflavonoid synthesis. This study provides useful insights into the molecular mechanism of S. alopecuroides in response to drought stress, and help develop biotechnological strategies to manipulate the levels of therapeutic specialized metabolites in S. alopecuroides.

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