Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation
文献类型: 外文期刊
作者: Wang, Lu 1 ; Cheng, Jianing 1 ; Bi, Shuangtian 1 ; Wang, Jinshu 1 ; Cheng, Xin 1 ; Liu, Shihang 1 ; Gao, Yue 1 ; Lan, Qingkuo 2 ; Shi, Xiaowei 2 ; Wang, Yong 2 ; Zhao, Xin 2 ; Qi, Xin 2 ; Xu, Shiyong 2 ; Wang, Che 1 ;
作者机构: 1.Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110866, Peoples R China
2.Tianjin Acad Agr Sci, Inst Germplasm Resource & Biotechnol, Tianjin 300384, Peoples R China
3.Tianjin Acad Agr Sci, State Key Lab Vegetable Biobreeding, Tianjin 300392, Peoples R China
关键词: ADF1; MYB30; actin filaments; thermal adaptation; Arabidopsis; Chinese cabbage
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.6; 五年影响因子:6.2 )
ISSN:
年卷期: 2023 年 24 卷 6 期
页码:
收录情况: SCI
摘要: Actin filaments are essential for plant adaptation to high temperatures. However, the molecular mechanisms of actin filaments in plant thermal adaptation remain unclear. Here, we found that the expression of Arabidopsis actin depolymerization factor 1 (AtADF1) was repressed by high temperatures. Compared with wild-type seedlings (WT), the mutation of AtADF1 and the overexpression of AtADF1 led to promoted and inhibited plant growth under high temperature conditions, respectively. Further, high temperatures induced the stability of actin filaments in plants. Compared with WT, Atadf1-1 mutant seedlings showed more stability of actin filaments under normal and high temperature conditions, while the AtADF1 overexpression seedlings showed the opposite results. Additionally, AtMYB30 directly bound to the promoter of AtADF1 at a known AtMYB30 binding site, AACAAAC, and promoted the transcription of AtADF1 under high temperature treatments. Genetic analysis further indicated that AtMYB30 regulated AtADF1 under high temperature treatments. Chinese cabbage ADF1 (BrADF1) was highly homologous with AtADF1. The expression of BrADF1 was inhibited by high temperatures. BrADF1 overexpression inhibited plant growth and reduced the percentage of actin cable and the average length of actin filaments in Arabidopsis, which were similar to those of AtADF1 overexpression seedlings. AtADF1 and BrADF1 also affected the expression of some key heat response genes. In conclusion, our results indicate that ADF1 plays an important role in plant thermal adaptation by blocking the high-temperature-induced stability of actin filaments and is directly regulated by MYB30.
- 相关文献
作者其他论文 更多>>
-
Lightweight fungal spore detection based on improved YOLOv5 in natural scenes
作者:Li, Kaiyu;Qiao, Chen;Zhu, Xinyi;Song, Yuzhaobi;Zhang, Lingxian;Zhang, Lingxian;Gao, Wei;Wang, Yong
关键词:Fungal spores; Object detection; YOLOv5; Attention mechanism
-
The Gray Mold Spore Detection of Cucumber Based on Microscopic Image and Deep Learning
作者:Li, Kaiyu;Zhu, Xinyi;Qiao, Chen;Zhang, Lingxian;Zhang, Lingxian;Gao, Wei;Wang, Yong
关键词:
-
Regulation of bta-miRNA29d-3p on Lipid Accumulation via GPAM in Bovine Mammary Epithelial Cells
作者:Zhao, Xin;Chen, Lili;Zhang, Man;Ma, Yi;Yao, Dawei;Zhao, Xin;Li, Jun;Zhao, Shuying
关键词:miR-29; lipid metabolism; GPAM; milk fat; cow mammary cells
-
Maleic hydrazide prompting growth and delaying senescence of mother frond in S. Polyrriza 7498
作者:Wang, Jiahuan;Gao, Tianyu;Hu, Hong;Sun, Ling;Ban, Shaoqiang;Tan, Xiaoyu;Li, Xiaoxue;Zhu, Mingyue;Zhu, Yerong;Wang, Yong;Tong, Yana
关键词:Maleic hydrazide; Mother frond; Growth; Cytokinin; Senescence; S; Polyrriza 7498
-
Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway
作者:Wang, Bo;Wang, Yiheng;Yu, Wancong;Lan, Qingkuo;Wang, Yong;Wang, Bo;Xie, Likun;Chen, Chengbin;Wang, Bo;Zhang, Yong
关键词:CRISPR-Cas9; cold stress; RNA-seq; photosynthesis; soluble sugar
-
Overexpression of the Phosphoserine Phosphatase-Encoding Gene (AtPSP1) Promotes Starch Accumulation in Lemna turionifera 5511 under Sulfur Deficiency
作者:Wang, Lei;Kuang, Yingying;Zheng, Siyu;Zhu, Yerong;Wang, Yong;Tong, Yana
关键词:starch accumulation; AtPSP1 overexpression; sulfur deficiency; Lemna turionifera 5511
-
Complete Genome Sequence Resource for Cercospora apii Causing Leaf Spot of Celery
作者:Yang, Lijuan;Gao, Wei;Zhang, Chunxiang;Huo, Jianfei;Wang, Yong;Gao, Guoxun;Wu, Feng
关键词:celery; Cercospora apii; genome; leaf spot