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Effect of ferric ions on Cronobacter sakazakii growth, biofilm formation, and swarming motility

文献类型： 外文期刊

作者： Wang, Yang 1 ; Ling, Na 1 ; Wang, Yaping 1 ; Ou, Dexin 1 ; Liang, Zhao 1 ; Li, Guoqing 2 ; Zhao, Hongyuan 2 ; Ye, Yingwang 1 ;

作者机构： 1.Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230601, Peoples R China

2.Anhui Acad Agr Sci, Inst Hort Res, Hefei 230031, Peoples R China

3.Anhui Acad Agr Sci, 40 Silihe Rd, Hefei 230031, Anhui, Peoples R China

4.Tunxi Rd 193, Hefei 230009, Anhui, Peoples R China

关键词： Cronobacter sakazakii; Iron; Biofilm; Swarming motility; Flagella; Transcriptomic analysis

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）

ISSN： 0168-1605

年卷期： 2024 年 408 卷

页码：

收录情况： SCI

摘要： Cronobacter sakazakii (C. sakazakii) is a common food-borne pathogen that induces meningitis, sepsis, and necrotizing enterocolitis, primarily in newborns and infants. Iron plays a pivotal role in the growth of cells and biofilm formation. However, the effects of hemin (ferric ion donor) on C. sakazakii cells are scarcely known. Here, we explored the effect of ferric ions on the growth of planktonic C. sakazakii, biofilm formation, and swarming motility by crystal violet staining (CVS), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and swarming assay. Our study demonstrated that ferric ions facilitated the growth of planktonic C. sakazakii, while hemin at concentrations ranging from 50 to 800 mu mol/L promoted biofilm for-mation and at concentrations between 50 and 200 mu mol/L enhanced the swarming motility of C. sakazakii. Furthermore, high hemin concentrations (400-800 mu mol/L) were found to reduce flagellar length, as confirmed by transmission electron microscopy (TEM). These findings indicated that ferric ions mediated the swarming motility of C. sakazakii by regulating flagellar assembly. Finally, transcriptomic analysis of C. sakazakii was performed at hemin concentrations of 0, 50, and 200 mu mol/L, which revealed that several genes associated with iron transport and metabolism, and flagellar assembly were essential for the survival of C. sakazakii under hemin treatment. Our findings revealed the molecular basis of ferric ions on C. sakazakii growth and biofilm formation, thus providing a novel perspective for its prevention and control.

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