Positional effects of double-stranded RNAs targeting ss-Actin gene affect RNA interference efficiency in Colorado potato beetle
文献类型: 外文期刊
作者: He, Wanwan 1 ; Xu, Wenbo 1 ; Fu, Kaiyun 3 ; Guo, Wenchao 4 ; Kim, Dae Sung 1 ; Zhang, Jiang 1 ;
作者机构: 1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China
2.Wuhan Acad Agr Sci, Wuhan 430070, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi 830000, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Microbial Applicat, Urumqi 830000, Peoples R China
关键词: Colorado potato beetle; RNAi; dsRNA; Positional effects; Foliar application; Potato
期刊名称:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:4.966; 五年影响因子:4.591 )
ISSN: 0048-3575
年卷期: 2022 年 184 卷
页码:
收录情况: SCI
摘要: Pesticide resistance in pests drives the development of RNA interference (RNAi)-based technology as a novel approach for pest control. To investigate the effects of the positional dependency of double-stranded RNAs (dsRNAs), we newly designed four different 200 bp dsRNAs targeting Colorado potato beetle (CPB) ss-Actin gene, termed as dsACT200-1 to dsACT200-4, to compare their insecticidal activity to CPB larvae together with our previously used 200 bp and 700 bp dsRNAs (dsACT200 and dsACT700), respectively (He et al., 2020a). Each of dsRNAs harbors different numbers of expected siRNAs predicted by sequence-based prediction platform, dsACT200 and dsACT200-2 have a relatively higher number of siRNA than other 200 bps dsRNAs. When CPB larvae were fed with in vitro synthesized dsRNA-painted potato leaves, all the tested dsRNAs showed significant effects to protect against CPB larvae. Combined with the survival rate of CPB larvae, ss-Actin gene expression level and the surviving CPB larvae weight, various positional dsRNAs from the same allele showed different plant protection activity against CPB larvae and partially correlated with the predicted siRNA numbers and distribution on the target sequence. This study suggests the specific allelic locus for rational dsRNA design triggering RNAi efficiency for target gene silencing is an essential factor in enhancing the insecticidal activity.
- 相关文献
作者其他论文 更多>>
-
The interaction of nicotinic acetylcholine receptor subunits Ld & alpha;3, Ld & alpha;8 and Ld & beta;1 with neonicotinoids in Colorado potato beetle, Leptinotarsa decemlineata
作者:Shi, Chengcheng;Tian, Yitong;Wang, Yaqi;Jiang, Weihua;Guo, Wenchao
关键词:Leptinotarsa decemlineata; Thiamethoxam; Imidacloprid; Nicotinic acetylcholine receptor; Gene expression; RNA interference
-
Chromosome-level genome assembly and annotation of the prickly nightshade Solanum rostratum Dunal
作者:Zhang, Yue;Yuan, Zhili;Song, Zhen;Wang, Zhonghui;Gao, Jinhui;Fu, Weidong;Zhang, Guoliang;Guo, Wenchao
关键词:
-
Analysis of the virulence, infection process, and extracellular enzyme activities of Aspergillus nomius against the Asian corn borer, Ostrinia furnacalis guenée (Lepidoptera: Crambidae)
作者:Wang, Xiaowu;Yuan, Zihan;Zhan, Faqiang;Zhou, Liuyan;Dai, Jinping;Wang, Zhifang;Xie, Yuqing;Yang, Xinping;Wang, Xiaowu;Ding, Xinhua;Jia, Zunzun;Fu, Kaiyun;Guo, Wenchao;Li, Haiqiang;Ding, Xinhua;Jia, Zunzun;Fu, Kaiyun;Guo, Wenchao;Li, Haiqiang
关键词:Aspergillus nomius; ostrinia furnacalis; lethal efficacy; infection processes; cuticle; extracellular enzymes activity
-
Colorado potato beetle exploits frass-associated bacteria to suppress defense responses in potato plants
作者:Gao, Zhou;Ju, Xueyang;Xue, Rongrong;Li, Qing;Tong, Lu;Song, Yuanyuan;Zeng, Rensen;Wang, Jie;Yang, Mingyu;Fu, Kaiyun;Guo, Wenchao
关键词:Colorado potato beetle; frass; potato; symbiotic bacteria; induced defense
-
CAP Analysis of the Distribution of the Introduced Bemisia tabaci (Hemiptera: Aleyrodidae) Species Complex in Xinjiang, China and the Southerly Expansion of the Mediterranean Species
作者:Jia, Zunzun;Fu, Kaiyun;Ahmat, Tursun;Ding, Xinhua;He, Jiang;Guo, Wenchao;Wang, Xiaowu;Jiang, Weihua
关键词:Bemisia tabaci; cryptic species; invasive species; genetic diversity
-
Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle
作者:He, Wanwan;Xu, Wenbo;Xu, Letian;Bock, Ralph;Zhang, Jiang;Bock, Ralph;Guo, Wenchao;Fu, Kaiyun
关键词:Colorado potato beetle; double-stranded RNA; pest control; plastid engineering; RNA interference; transplastomic plants
-
Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle
作者:He, Wanwan;Xu, Wenbo;Zhang, Jiang;Fu, Kaiyun;Guo, Wenchao
关键词:RNA interference; double-stranded RNA; sequence mutation; resistance development; Colorado potato beetle