文献类型： 外文期刊

作者： Gao, De-Zhen ¹ ; Liu, Guo-Hua ² ; Wang, Guang-Lei ³ ; Zhu, Xing-Quan ¹ ; Wang, Chun-Ren ¹ ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Heilongjiang, Peoples R China

2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Gansu, Peoples R China

3.Xinjiang Acad Anim Sci, Vet Res Inst, Urumqi, Peoples R China

关键词： Gasterophilus intestinalis;genetic variation;mitochondrial DNA;phylogenetic analysis

期刊名称：MITOCHONDRIAL DNA PART A （ 影响因子：1.514； 五年影响因子：0.707 ）

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收录情况： SCI

摘要： Horse gastrointestinal myiasis caused by larvae of Gasterophilus spp. has a worldwide distribution. However, little information is available on the genetic variation of Gasterophilus spp. at the molecular level. In the present study, sequence variation was examined in three mitochondrial (mt) genes, namely mt cytochrome c oxidase subunit 1 (coxl), NADH dehydrogenase subunits 5 (nadS) and large subunit ribosomal RNA (rrnL), among G. intestinalis isolates originating from Heilongjiang Provience and Xinjiang Uygur Autonomous Region, China. The complete coxl, nadS, and rrnL gene sequences were amplified by PCR separately from adult G. intestinalis individuals and the amplicons were sequenced from both directions. The size of the sequences of coxl, nad5, and rrnL genes was 1539 bp, 1717 bp, and 1321 bp, respectively. The A + T contents of the sequences were 62.7-63.9% (coxl), 70-70.7% (nad5), and 74.5-74.9% (rrnL). The intra-specific sequence variations within G. intestinalis were 0.1-3.1% for coxl, 0.4-3.4% for nadS, and 0.1-1.5% for rrnL. Phylogenetic analyses based on the coxl sequences using the Bayesian inference indicated that all the G. intestinalis isolates grouped together with high statistical support. These findings demonstrated clearly the usefulness of mt coxl, nadS, and rrnL sequences for the molecular identification and for studying population genetics of G. intestinalis in horses.