文献类型： 外文期刊

作者： Jiang, Wenhui ¹ ; Jiang, Qinqin ¹ ; Shui, Zhijie ¹ ; An, Peipei ¹ ; Shi, Shandang ¹ ; Liu, Tianxiang ¹ ; Zhang, Hanbing ¹ ; Huang, Shuyi ¹ ; Jing, Bing ¹ ; Xiao, Enshi ¹ ; Quan, Li ¹ ; Liu, Jixia ³ ; Wang, Zhonghua ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

2.Chinese Acad Agr Sci CAAS, Guangdong Lab Lingnan Modern Agr, Agr Gen Inst Shenzhen, Genome Anal Lab,Minist Agr, Shenzhen 518120, Peoples R China

3.Ningxia Acad Agr & Forestry Sci, Crop Res Inst, Yinchuan 750002, Ningxia, Peoples R China

关键词： Sunflower; Flavonol; Anthocyanin; MYB; BHLH

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2024 年 338 卷

页码：

收录情况： SCI

摘要： Sunflowers are well-known ornamental plants, while sunflowers with red corolla are rare and the mechanisms underlying red coloration remain unclear. Here, a comprehensive analysis of metabolomics and transcriptomics on flavonoid pathway was performed to investigate the molecular mechanisms underlying the differential color formation between red sunflower Pc103 and two yellow sunflowers (Yr17 and Y35). Targeted metabolomic analysis revealed higher anthocyanin levels but lower flavonol content in Pc103 compared to the yellow cultivars. RNA-sequencing and phylogenetic analysis identified multiple genes involved in the flavonoid pathway, including series of structural genes and three MYB and bHLH genes. Specifically, HaMYBA and HabHLH1 were up-regulated in Pc103, whereas HaMYBF exhibited reduced expression. HaMYBA was found to interact with HabHLH1 in vivo and in vitro, while HaMYBF does not. Transient expression analysis further revealed that HabHLH1 and HaMYBA cooperatively regulate increased expression of dihydroflavonol 4-reductase (DFR), leading to anthocyanin accumulation. On the other hand, ectopic expression of HaMYBF independently modulates flavonol synthase (FLS) expression, but hindered anthocyanin production. Collectively, our findings suggest that the up-regulation of HaMYBA and HabHLH1, as well as the down-regulation of HaMYBF, contribute to the red coloration in Pc103. It offers a theoretical basis for improving sunflower color through genetic engineering.