文献类型： 外文期刊
作者： Luo, Man 1 ; Wang, Lin 1 ; Xiao, Cheng 4 ; Zhou, Mengsi 2 ; Li, Minghui 2 ; Li, Hongjuan 2 ;
作者机构： 1.Zhengzhou Univ, Metab Dis Res Ctr, Zhengzhou Cent Hosp, Zhengzhou, Peoples R China
2.Zhengzhou Univ, Dept Obstet & Gynecol, Zhengzhou Cent Hosp, 16 Tongbai North Rd, Zhengzhou 450001, Peoples R China
3.Zhengzhou Univ, Sch Basic Med Sci, Zhengzhou, Peoples R China
4.Jilin Acad Agr Sci, Inst Anim Biotechnol, Gongzhuling, Peoples R China
关键词： miR136; differentiation; proliferation; sheep preadipocytes; intramuscular fat
期刊名称：ADIPOCYTE （ 影响因子：3.3； 五年影响因子：4.0 ）
ISSN： 2162-3945
年卷期： 2023 年 12 卷 1 期
页码：
收录情况： SCI
摘要： Low meat performance is the defect of Small Tail Han sheep. Intramuscular fat affects meat quality and largely determined by adipogenesis. In previous study, miR136 was showed one of differentially expressed microRNAs between preadipocytes and mature adipocytes of Small Tail Han sheep but its role in adipogenesis is still not elucidated. Here, we investigated the effect of miR136 on adipogenesis and the underlying mechanism. qPCR data showed that miR136 level increased with preadipocytes proliferation while declined with preadipocytes differentiation. Moreover, miR136 mimics blocked lipid droplet formation, reduced lipid content and triglyceride accumulation while miR136 inhibitor showed the opposite effects, revealing that miR136 promoted preadipocytes proliferation but inhibited preadipocytes differentiation. Bioinformatics and biochemical validation manifested that PPARGC1B was a target of miR136. Furthermore, miR136 mimics decreased PPAR gamma and C/EBP alpha expression accompanied by PPARGC1B expression descending. Reverse effects were observed with miR136 inhibitor. Besides, overexpression of miR136 elevated IGF1 expression. Collectively, our data first exhibited a regulatory role of miR136 in adipogenesis, which is promoting preadipocytes proliferation through elevating IGF1 expression while inhibiting preadipocytes differentiation through targeting PPARGC1B and further declined PPAR gamma and C/EBP alpha expression. The modulation of PPARGC1B by miR136 may provide a new potential target for increasing intramuscular fat.
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