Optimization of Submerged Culture Parameters of the Aphid Pathogenic Fungus Fusarium equiseti Based on Sporulation and Mycelial Biomass
文献类型: 外文期刊
作者: Zhao, Xueyi 1 ; Chai, Junfa 1 ; Wang, Fang 2 ; Jia, Yanxia 1 ;
作者机构: 1.Ningxia Univ, Coll Agr, Yinchuan 750021, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan 750002, Peoples R China
关键词: Fusarium equiseti; optimal submerged culture conditions; response surface methodology; box-behnken design; Myzus persicae
期刊名称:MICROORGANISMS ( 影响因子:4.5; 五年影响因子:4.8 )
ISSN:
年卷期: 2023 年 11 卷 1 期
页码:
收录情况: SCI
摘要: Fusarium equiseti (JMF-01), as an entomopathogenic fungus, can effectively control agricultural pests and has the potential to be a biocontrol agent. To promote mycelial growth and sporulation, we investigated the optimal submerged culture conditions for F. equiseti. In this study, we used the single-factor method and Box-Behnken design and determined the virulence of the submerged culture against Myzus persicae after optimization. As a result, the highly significant factors affecting the spore concentration of strain JMF-01 were the primary inoculum density and the initial pH, and the highly significant factor affecting the mycelial biomass was the medium-to-flask ratio. The highest mycelial biomass value was 0.35 g when the incubation time was 5.68 days, the initial pH was 5.11, the medium-to-flask ratio was 0.43, and 1 mL of the primary inoculum with spore density of 0.97 x 10(7) conidia/mL was added. When the incubation time was 6.32 days, the initial pH was 4.46, the medium-to-flask ratio was 0.35, the primary inoculum density was 1.32 x 10(7) conidia/mL of 1 mL, and the highest spore concentration of 6.49 x 10(8) blastospores/mL was obtained. Compared with the unoptimized medium conditions, the optimized submerged culture had the highest mycelial biomass and spore concentration, which were 3.46 and 2.06 times higher, respectively. The optimized submerged culture was highly pathogenic toward M. persicae, reaching a 95% mortality rate. Our results provide optimal submerged culture conditions for F. equiseti and lay the basis for later research to expand production for pest control.
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