Development of transgenic composite Stylosanthes plants to study root growth regulated by a beta-expansin gene, SgEXPB1, under phosphorus deficiency
文献类型: 外文期刊
作者: Wang, Linjie 1 ; Wang, Wenqiang 1 ; Miao, Ye 2 ; Peters, Michael 3 ; Schultze-Kraft, Rainer 3 ; Liu, Guodao 1 ; Chen, Zhijian 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Crop Genet Resources, Key Lab Trop Crops Germplasm Resources Genet Impro, Haikou 571101, Peoples R China
2.Hainan Univ, Coll Trop Crops, Haikou 570110, Peoples R China
3.Alliance Biovers Int & Int Ctr, Trop Agr, Cali 763537, Colombia
关键词: Composite plants; Hairy roots; Agrobacterium rhizogenes; beta-Expansin gene; P deficiency; Stylosanthes guianensis
期刊名称:PLANT CELL REPORTS ( 影响因子:6.2; 五年影响因子:5.9 )
ISSN: 0721-7714
年卷期: 2023 年 42 卷 3 期
页码:
收录情况: SCI
摘要: Stylo (Stylosanthes spp.) is an important forage legume widely applied in agricultural systems in the tropics. Due to the recalcitrance of stylo genetic transformation, functional characterization of candidate genes involved in stylo root growth is limited. This study established an efficient procedure for Agrobacterium rhizogenes-mediated transformation for generating transgenic composite plants of S. guianensis cultivar 'Reyan No. 5'. Results showed that composite stylo plants with transgenic hairy roots were efficiently generated by A. rhizogenes strains K599 and Arqual, infecting the residual hypocotyl at 1.0 cm of length below the cotyledon leaves of 9-d-old seedlings, leading to a high transformation efficiency of > 95% based on histochemical beta-glucuronidase (GUS) staining. Notably, 100% of GUS staining-positive hairy roots can be achieved per composite stylo plant. Subsequently, SgEXPB1, a beta-expansin gene up-regulated by phosphorus (P) deficiency in stylo roots, was successfully overexpressed in hairy roots. Analysis of hairy roots showed that root growth and P concentration in the transgenic composite plants were increased by SgEXPB1 overexpression under low-P treatment. Taken together, a highly efficient A. rhizogenes-mediated transformation procedure for generating composite stylo plants was established to study the function of SgEXPB1, revealing that this gene is involved in stylo root growth during P deficiency.
- 相关文献
作者其他论文 更多>>
-
Characterization of phosphate transporter genes and the function of SgPT1 involved in phosphate uptake in Stylosanthes guianensis
作者:An, Na;Chen, Zhijian;An, Na;Huang, Jie;Liu, Pandao;Liu, Guodao;Chen, Zhijian;Xue, Yingbin;Zhu, Shengnan
关键词:P deficiency; Pi transporter; Pi uptake; Gene expression; Stylosanthes guianensis
-
Genome-Wide Mining of the Tandem Duplicated Type III Polyketide Synthases and Their Expression, Structure Analysis of Senna tora
作者:Cai, Zeping;Fang, Ting;Zhao, Xingkun;Zhou, Chaoye;Zhao, Xingkun;Zhou, Chaoye;Liu, Guodao;Luo, Jiajia
关键词:chalcone synthase-like gene; expression and structure analysis; Senna tora; tandem duplication; type III polyketide synthase
-
Cover legumes promote the growth of young rubber trees by increasing organic carbon and organic nitrogen content in the soil
作者:Hu, An;Huang, Dongfen;Duan, Qianwen;Zhou, Yan;Liu, Guodao;Huan, Hengfu;Zhou, Yan
关键词:Perennial cover crops; Mineral N; Organic N fractions; Labile organic carbon; Rubber plantations
-
Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes
作者:Chen, Zhijian;Wang, Linjie;Liu, Guodao;Lin, Yan;Cardoso, Juan Andres;Rao, Idupulapati M.;Zhu, Shengnan;Rao, Idupulapati M.;Lin, Yan
关键词:phosphorus deficiency; root morphology; root architecture; root traits; Pi uptake; PSI genes
-
Natural vegetation regeneration facilitated soil organic carbon sequestration and microbial community stability in the degraded karst ecosystem
作者:Cheng, Hanting;Zhou, Xiaohui;Li, Qinfen;Cheng, Hanting;Zhou, Xiaohui;Li, Qinfen;Cheng, Hanting;Zhou, Xiaohui;Li, Qinfen;Dong, Rongshu;Liu, Guodao;Wang, Xiaomin;Li, Qinfen;Li, Qinfen;Li, Qinfen
关键词:Restoration strategies; Labile SOC fractions; Soil microbial communities; Degraded karst ecosystem
-
Analysis of seed production and seed shattering in a new artificial grassland forage: pigeon pea
作者:Li, Xinyong;Sheng, Wei;Dong, Qianzhen;Huang, Rui;Dong, Rongshu;Liu, Guodao;Ding, Xipeng;Sheng, Wei;Dong, Qianzhen;Zhang, Jingwen
关键词:pigeon pea; seed yield; yield component; structural equation model; seed shattering; abscission layer
-
Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots
作者:Liu, Xiaomei;Liu, Xiaomei;Tian, Jing;Sun, Lili;Liu, Xiaomei;Liu, Guodao
关键词:tea plant; phosphorus; root growth; multi-omics