Isolation, Identification, and Genetic Phylogenetic Analysis of Two Different Genotypes of Bovine Parainfluenza 3 Virus in China
文献类型： 外文期刊
作者： Wang, Xu 1 ; Hu, Jianjun 2 ; Meng, Fanyan 1 ; Cao, Yiheng 1 ; Wang, Zijie 1 ; Zhang, Qianyi 4 ; Zhang, Qian 1 ; Zhang, Xingxing 1 ; Han, Mengli 1 ; Wu, Tongzhong 1 ; Zhong, Fagang 1 ; Huang, Xin 1 ;
作者机构： 1.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Genet Improvement & Hlth Breeding S, Shihezi 832000, Peoples R China
2.Tarim Univ, Coll Anim Sci & Technol, Aral 843300, Peoples R China
3.China Anim Husb Ind Co Ltd, Beijing 100070, Peoples R China
4.China Vet Drug Control Inst, Beijing 100081, Peoples R China
关键词： bovine parainfluenza virus type 3; genetic phylogenetic analysis; virus identification; indirect immunofluorescence; virus isolation; next-generation sequencing
期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）
ISSN：
年卷期： 2022 年 14 卷 10 期
页码：
收录情况： SCI
摘要： Bovine parainfluenza virus 3 (BPIV3) is one of several viruses that contribute to bovine respiratory disease complex (BRDC). During this study, isolation of BPIV3 was attempted from 20 PCR-positive swabs by Madin-Darby Bovine Kidney (MDBK) cells. Nine samples showed obvious cytopathic lesions identified as BPIV3 by reverse-transcription polymerase chain reaction amplification and sequencing. The genomes of isolates XJ21032-1 and XJ20055-3 were sequenced using Illumina sequencing technology and determined to have lengths of 15,512 bp and 15,479 bp, respectively. Phylogenetic analysis revealed that isolate XJ21032-1 was genotype B, and isolate XJ20055-3 was genotype C. In addition, the two isolates had multiple amino acid changes in nucleocapsid protein, fusion protein, and hemagglutinin/neuraminidase, major antigenic proteins. This allows the further recognition of the presence of BPIV3 type B in Chinese cattle herds. We hope this will help trace the origin of BPIV3, improve the understanding of differences between genotypes, and provide data support for vaccine development.
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