Evaluation of efficacy and mechanism of Bacillus velezensis CB13 for controlling peanut stem rot caused by Sclerotium rolfsii
文献类型: 外文期刊
作者: Jia, Shu 1 ; Song, Ce 2 ; Dong, Hai 3 ; Yang, Xujie 1 ; Li, Xinghai 1 ; Ji, Mingshan 1 ; Chu, Jin 3 ;
作者机构: 1.Shenyang Agr Univ, Coll Plant Protect, Shenyang, Peoples R China
2.Sericultural Res Inst Liaoning Prov, Fengcheng, Peoples R China
3.Liaoning Acad Agr Sci, Inst Plant Protect, Shenyang, Peoples R China
关键词: Bacillus velezensis CB13; Sclerotium rolfsii; peanut stem rot; defense enzyme; colonization; soil rhizosphere microbiome; control efficiency
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.2; 五年影响因子:6.2 )
ISSN:
年卷期: 2023 年 14 卷
页码:
收录情况: SCI
摘要: Peanut stem rot, caused by Sclerotium rolfsii, considerably affects crop productivity. Application of chemical fungicides harms the environment and induces drug resistance. Biological agents are valid and eco-friendly alternatives to chemical fungicides. Bacillus spp. are important biocontrol agents that are now widely used against several plant diseases. This study aimed to evaluate the efficacy and mechanism of a potential biocontrol agent Bacillus sp. for controlling peanut stem rot caused by S. rolfsii. Here, we isolated a strain of Bacillus from pig biogas slurry that considerably inhibits the radial growth of S. rolfsii. The strain CB13 was identified as Bacillus velezensis on the basis of morphological, physiological, biochemical characteristics and phylogenetic trees based on the 16S rDNA and gyrA, gyrB, and rpoB gene sequences. The biocontrol efficacy of CB13 was evaluated on the basis of colonization ability, induction of defense enzyme activity, and soil microbial diversity. The control efficiencies of B. velezensis CB13-impregnated seeds in four pot experiments were 65.44, 73.33, 85.13, and 94.92%. Root colonization was confirmed through green fluorescent protein (GFP)-tagging experiments. The CB13-GFP strain was detected in peanut root and rhizosphere soil, at 10(4) and 10(8) CFU/g, respectively, after 50 days. Furthermore, B. velezensis CB13 enhanced the defense response against S. rolfsii infection by inducing defense enzyme activity. MiSeq sequencing revealed a shift in the rhizosphere bacterial and fungal communities in peanuts treated with B. velezensis CB13. Specifically, the treatment enhanced disease resistance by increasing the diversity of soil bacterial communities in peanut roots, increasing the abundance of beneficial communities, and promoting soil fertility. Additionally, real-time quantitative polymerase chain reaction results showed that B. velezensis CB13 stably colonized or increased the content of Bacillus spp. in the soil and effectively inhibited S. rolfsii proliferation in soil. These findings indicate that B. velezensis CB13 is a promising agent for the biocontrol of peanut stem rot.
- 相关文献
作者其他论文 更多>>
-
Calcineurin B-like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice
作者:Chen, Huan;Lin, Qiujun;Li, Zhuo;Mei, Qiong;Xuan, Yuanhu;Lin, Qiujun;Chu, Jin;Dong, Hai;Mei, Qiong;Xuan, Yuanhu
关键词:CIPK31; homeostasis; resistance; rice; ROS; sheath blight
-
A Genome-Wide Comparison of Rice False Smut Fungus Villosiclava virens Albino Strain LN02 Reveals the Genetic Diversity of Secondary Metabolites and the Cause of Albinism
作者:Xue, Mengyao;Zhao, Siji;Gu, Gan;Xu, Dan;Zhang, Xuping;Hou, Xuwen;Lai, Daowan;Zhou, Ligang;Miao, Jiankun;Dong, Hai;Hu, Dongwei
关键词:rice false smut; Villosiclava virens (Ustilaginoidea virens); albino strain; genome-wide comparison; ustilaginoidins; sorbicillinoids
-
Occurrence and mechanism of target-site resistance to bensulfuron-methyl in Monochoria korsakowii from China
作者:Du, Ying;Deng, Yunyan;Zhang, Lulu;Bai, Tianlang;Ji, Mingshan;Wang, Minlong;Chen, Yan
关键词:Monochoria korsakowii; Acetohydroxyacid synthase; Sulfonylurea herbicides; Resistance mutations; Enzyme functionality; Gene expression
-
An Examination of Regional Variations in Pesticide Usage and Grain Yield in China Before and After the Double Reduction Policy?s Adoption
作者:Yang, Mei;Chu, Jin;Li, Zhiqiang;Yu, Fengquan;Sun, Fuyu;Liu, Xinyu
关键词:double reduction policy; pesticide usage; grain yield; regional difference
-
CBL-interacting protein kinase 31 regulates rice resistance to blast disease by modulating cellular potassium levels
作者:Lin, Qiu Jun;Li, Zhi Min;Wu, Xian Xin;Sun, Qian;Xuan, Yuan Hu;Kumar, Vikranth;Chu, Jin;Dong, Hai
关键词:CBL; CIPK31; Defense; Rice blast; Potassium
-
Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease
作者:Chu, Jin;Xu, Han;Dong, Hai;Xuan, Yuan Hu
关键词:KLP; Sheath blight disease; Transcription activation; Defense; Rice
-
Protein Phosphatase 2A Catalytic Subunit PP2A-1 Enhances Rice Resistance to Sheath Blight Disease
作者:Lin, Qiu Jun;Yuan, De Peng;Li, Zhi Min;Mei, Qiong;Xuan, Yuan Hu;Chu, Jin;Kumar, Vikranth
关键词:PP2A-1; sheath blight; resistance; enhance; rice