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Co-expression of four penaeidins in transgenic rice seeds: an alternative strategy for substitute antibiotic agricultural products

文献类型： 外文期刊

作者： Song, Xinyuan 1 ; Qiao, Yu 1 ; Ma, Jian 2 ; Zhang, Xue 1 ; Liu, Jie 1 ; Xin, Wen 3 ; Xing, Shaochen 1 ; Wang, Yunpeng 1 ;

作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Peoples R China

2.Jilin Agr Univ, Fac Agron, Changchun 130000, Peoples R China

3.Beijing TransGen Biotech Co Ltd, Beijing 100192, Peoples R China

关键词： Penaeidin; Antimicrobial peptide; Transgenic rice; Genetically modified crops

期刊名称：TRANSGENIC RESEARCH （ 影响因子：3.0； 五年影响因子：2.7 ）

ISSN： 0962-8819

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The co-expression of multiple antimicrobial peptides (AMPs) in genetically modified (GM) crops can give plants a broader antibacterial spectrum and lower the pathogen risk of drug resistance. Therefore, four penaeidins (shrimp-derived AMPs) were fused and encoded in an artificial gene (PEN1234), driven by the seed-specific promoter Pzein, with the aim of co-expression in seeds of transgenic rice. The resistant rice plants, acquired via Agrobacterium-mediated transformation and glufosinate screening, were identified by PCR and the modified disk-diffusion method, and eight GM lines with high AMP content in the seeds were obtained. Among them, the PenOs017 line had the largest penaeidin content, at approximately 251-300 & mu;g/g in seeds and 15-47 & mu;g/g in roots and leaves. The AMPs in the seeds kept their antibacterial properties even after the seed had been boiled in hot water and could significantly inhibit the growth of methicillin-resistant Staphylococcus aureus, and AMPs in the leaves could effectively inhibit Xanthomonas oryzae pv. Oryzae. The results indicate that PenOs017 seeds containing AMPs are an ideal raw-material candidate for antibiotic-free food and feed, and may require fewer petrochemical fungicides or bactericides for disease control during cultivation than conventional rice.

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