Development of a reverse genetics system for Japanese encephalitis virus strain SA14-14-2
文献类型: 外文期刊
作者: Li, Guohua 1 ; Jin, Hongli 2 ; Nie, Xin 8 ; Zhao, Yongkun 2 ; Feng, Na 2 ; Cao, Zongxi 4 ; Tan, Shuyi 4 ; Zhang, Bo 5 ; Gai, 1 ;
作者机构: 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Peoples R China
2.Acad Mil Med Sci, Inst Mil Vet, Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Jilin, Peoples R China
3.Jilin Univ, Coll Vet Med, Minist Educ, Key Lab Zoonosis Res, Changchun 130062, Jilin, Peoples R China
4.Hainan Acad Agr Sci, Inst Anim Husb & Vet Med, Hainan Academician Workstn, Haikou 571100, Hainan, Peoples R China
5.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China
6.Natl Res Ctr Exot Anim Dis, Anim Hlth & Epidemiol Ctr, Qingdao 266032, Shandong, Peoples R China
7.Yangzhou Univ, Jiangsu Co Innovat Ctr Prevent & Control Importan, Yangzhou 225009, Jiangsu, Peoples R China
8.Peoples Liberat Army, 65316 Troops, Wafangdian 116300, Peoples R China
9.Jilin Agricutural Univ, Coll Anim Sci & Technol, Changchun 130118, Jilin, Peoples R China
关键词: Japanese encephalitis virus; Infectious clone; Intron; T7 terminator
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN: 0920-8569
年卷期: 2019 年 55 卷 4 期
页码:
收录情况: SCI
摘要: Japanese encephalitis virus SA14-14-2 (JEV SA14-14-2) is a widely used vaccine in China and other southeastern countries to prevent Japanese encephalitis in children. In this study, a stable infectious cDNA clone of JEV SA14-14-2 with a low copy number pACYC177 vector dependent on the T7 promoter and T7 terminator was developed. Two introns were inserted into the capsid gene and envelope gene of JEV cDNA for gene stability. Hepatitis delta virus ribozyme (HDVr) was engineered into the 3 ' UTR cDNA of JEV for authentic 3 ' UTR transcription. The rescued virus showed biological properties indistinguishable from those of the parent strain (JEV SA14-14-2). The establishment of a JEV SA14-14-2 reverse genetics system lays the foundation for the further development of other flavivirus vaccines and viral pathogenesis studies.
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