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Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application

文献类型： 外文期刊

作者： Li, Zhonghe 1 ; Gao, Xingai 1 ; Li, Ming 2 ; Yan, Qiuliang 1 ; Zhang, Nan 1 ; Yu, Boyang 1 ; Zhang, Bimi 3 ; Zhang, Shuying 4 ; Helal, Mohamed H. 5 ; Ali, Ola A. Abu 6 ; Nassan, Mohamed A. 7 ; Qyyum, Muhammad Abdul 8 ; Asif, Saira 9 ; Bokhari, Awais 9 ;

作者机构： 1.Jilin Acad Agr Sci, Changchun 130033, Peoples R China

2.Jilin Jianzhu Univ, Changchun 130118, Peoples R China

3.Econ Management Inst Jilin Prov, Changchun 130012, Peoples R China

4.Anim Dis Prevent & Control Ctr Jilin Prov, Changchun, Peoples R China

5.Northern Border Univ, Fac Arts & Sci, Dept Chem, PO 840, Rafha 91911, Saudi Arabia

6.Taif Univ, Coll Sci, Dept Chem, POB 11099, Taif 21944, Saudi Arabia

7.Taif Univ, Turabah Univ Coll, Dept Clin Lab Sci, POB 11099, Taif 21944, Saudi Arabia

8.Sultan Qaboos Univ, Dept Petro & Chem Engn, Muscat, Oman

9.Brno Univ Technol, Fac Mech Engn, Sustainable Proc Integrat Lab, SPIL,NETME Ctr,VUT Brno, Tech 2896 2, Brno 61600, Czech Republic

10.COMSATS Univ Islamabad CUI, Dept Chem Engn, Lahore Campus, Lahore 54000, Punjab, Pakistan

关键词： Fluorescent biosensor; Steroid hormone; EGFP-Tagged; Sediment; Soil; Water

期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：8.431； 五年影响因子：8.399 ）

ISSN： 0013-9351

年卷期： 2022 年 215 卷

页码：

收录情况： SCI

摘要： Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.

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