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OsPML2, a chloroplast envelope localized transporter is involved in manganese homeostasis in rice

文献类型： 外文期刊

作者： Zou, Yu 1 ; Xu, Ending 1 ; Fan, Ye 2 ; Zhang, Peijiang 1 ; Zhang, Wei 2 ; Chen, Xi 2 ;

作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Hefei 230031, Anhui, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Jiangsu, Peoples R China

关键词： Chloroplast; Envelope; Manganese; Photosynthesis; Rice; Transporter

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 203 卷

页码：

收录情况： SCI

摘要： Manganese (Mn), a vital element, plays crucial roles in various biochemical and physiological processes by serving as an essential cofactor for numerous enzymes and acting as a catalytically active metal within biological clusters. In this study, we investigate the role of PHOTOSYNTHESIS-AFFECTED MUTANT 71-LIKE 2 (OsPML2), a member of the UNCHARACTERIZED PROTEIN FAMILY 0016 (UPF0016) family, in regulating Mn homeostasis in rice. OsPML2 was highly expressed in young leaves, ovaries, and stigmas. Cross sections from young leaves revealed that OsPML2 was mainly expressed in the phloem region and mesophyll cells. Furthermore, heterologous expression of OsPML2 restored the growth of Mn uptake-defective yeast strain Delta smf1 under Mn-limited conditions. Subcellular localization analysis demonstrated that OsPML2 was specifically localized in the chloroplast envelope. Knockdown of OsPML2 resulted in reduced chloroplast Mn content, significantly affecting plant growth under Mn deficiency. Furthermore, analysis of isolated thylakoid membranes using blue native gels indicated a compromised accumulation of photosystem II (PSII) complexes in OsPML2 knockdown lines. Additionally, grain yield, grain length, and width were significantly reduced in OsPML2 knockdown plants. Collectively, our findings provide insights into the transport function of OsPML2, which facilitates Mn transport from the cytosol to chloroplast stroma and influences the accumulation of PSII complexes in rice.

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