文献类型： 外文期刊

作者： Chen, Zeng-Liang ² ; Huang, Cong ³ ; Li, Xi-Sheng ² ; Li, Guo-Cheng ⁴ ; Yu, Ting-Hong ² ; Fu, Guan-Jun ³ ; Zhang, Xue ³ ; Song, Ce ² ; Bai, Peng-Hua ⁶ ; Cao, Li ¹ ; Qian, Wan-Qiang ³ ; Wan, Fang-Hao ³ ; Han, Ri-Chou ¹ ; Tang, Rui ¹ ;

作者机构： 1.Guangdong Acad Sci, Inst Zool, Guangdong Key Lab Anim Conservat & Resource Utili, Guangdong Publ Lab Wild Anim Conservat & Utilizat, Guangzhou 510260, Peoples R China

2.Sericultural Inst Liaoning Prov, Fengcheng, Peoples R China

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr,Minist Agr,Genom, Shenzhen, Peoples R China

4.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China

5.Qingdao Agr Univ, Coll Plant Hlth & Med, Qingdao, Peoples R China

6.Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin, Peoples R China

7.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China

关键词： Calosoma maximoviczi; olfactory reception; transcriptome; expression profile; molecular docking

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.462； 五年影响因子：4.688 ）

ISSN： 1526-498X

年卷期： 2022 年 78 卷 6 期

页码：

收录情况： SCI

摘要： BACKGROUND The black carabid beetle Calosoma maximoviczi is a successful predator that serves as both a beneficial insect and a severe threat to economic herbivores. Its hunting technique relies heavily on olfaction, but the underlying mechanism has not been studied. Here, we report the electrophysiological, ecological and molecular traits of bioactive components identified from a comprehensive panel of natural odorants in the beetle-prey-plant system. The aim of this work was to investigate olfactory perceptions and their influence on the behaviours of C. maximoviczi. RESULTS Among the 200 identified volatiles, 18 were concentrated in beetle and prey samples, and 14 were concentrated in plants. Insect feeding damage to plants led to a shift in the emission fingerprint. Twelve volatiles were selected using successive electrophysiological tests. Field trials showed that significant sex differences existed when trapping with a single chemical or chemical mixture. Expression profiles indicated that sex-biased catches were related to the expression of 15 annotated CmaxOBPs and 40 CmaxORs across 12 chemosensory organs. In silico evaluations were conducted with 16 CmaxORs using modelling and docking. Better recognition was predicted for the pairs CmaxOR5-(Z)-3-hexenyl acetate, CmaxOR6-beta-caryophyllene, CmaxOR18-(E)-beta-ocimene and CmaxOR18-tetradecane, with higher binding affinity and a suitable binding pocket. Lastly, 168Y in CmaxOR6 and 142Y in CmaxOR18 were predicted as key amino acid residues for binding beta-caryophyllene and tetradecane, respectively. CONCLUSION This work provides an example pipeline for de novo investigation in C. maximoviczi baits and the underlying olfactory perceptions. The results will benefit the future development of trapping-based integrated pest management strategies and the deorphanization of odorant receptors in ground beetles. (c) 2022 Society of Chemical Industry.