Impacts of citric acid concentration and pH value on mechanism and rheological properties of cold-set whey protein fibrils hydrogels
文献类型: 外文期刊
作者: Wang, Zhiming 1 ; Deng, Yuanyuan 1 ; Zhang, Yan 1 ; Wei, Zhencheng 1 ; Wan, Zhili 3 ; Li, Chao 3 ; Tang, Xiaojun 1 ; Zhao, Zhihao 1 ; Zhou, Pengfei 1 ; Li, Ping 1 ; Liu, Guang 1 ; Zhang, Mingwei 1 ;
作者机构: 1.Minist Agr & Rural Affairs, Sericultural & Agrifood Res Inst, Key Lab Funct Foods, Guangdong Key Lab Agr Prod Proc,Guangdong Acad Agr, Guangzhou 510610, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
3.South China Univ Technol, Lab Food Prot & Colloids, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China
4.Food Lab Zhongyuan, Luohe 462300, Henan, Peoples R China
关键词: Amyloid fibrils; Protein interactions; Large amplitude oscillatory shear; Oral tribology
期刊名称:LWT-FOOD SCIENCE AND TECHNOLOGY ( 影响因子:6.0; 五年影响因子:6.0 )
ISSN: 0023-6438
年卷期: 2023 年 183 卷
页码:
收录情况: SCI
摘要: Whey protein fibrils (WFs) gelation is dependent on protein conformation which can be affected by citric acid (CA) and pH, but the related mechanism is unclear. CA concentrations (0-400 mmol/L) were applied to induce WFs gelation at acidic (pH 2), neutral (pH 7) and alkaline (pH 10) conditions, to investigate how CA concen-tration and pH value co-affect the mechanism and rheological properties of cold-set WFs hydrogels. CA promoted hydrophobic interactions of WFs molecules. At acidic condition, CA facilitated forming hydrogen bonds within WFs hydrogels in a level-dependent manner, high CA concentration was beneficial for gelation. At non-acidic condition, CA induced covalent cross-linking of WFs molecules by amide bonds and disulfide bonds, resulting in higher modulus of neutral WFs hydrogels. However, it also increases their sensitivity to deformation by reducing WF molecular flexibility. Excessive cross-linking induced by high CA concentration (400 mmol/L) was detrimental to gels formed at non-acidic condition. Despite the differences in gelation mechanism, the three types of WFs hydrogels exhibited similar physical changes and mouthfeel during oral processing (friction coefficient of 0.2-0.35). This study provided new insights into the gelation mechanism of CA-mediated cold-set WFs hydrogels and into designing an easy swallowing soft food gel based on WFs.
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