The cold-stress responsive gene DREB1A involved in low-temperature tolerance in Xinjiang wild walnut
文献类型: 外文期刊
作者: Han, Liqun 1 ; Ma, Kai 2 ; Zhao, Yu 2 ; Mei, Chuang 2 ; Mamat, Aisajan 2 ; Wang, Jixun 2 ; Qin, Ling 1 ; He, Tianming 1 ;
作者机构: 1.Xinjiang Agr Univ, Coll Hort, Urumqi, Peoples R China
2.Xinjiang Acad Agr Sci, Inst Hort Crops, Key Lab Genome Res & Genet Improvement Xinjiang Ch, Xinjiang Fruit Sci Expt Stn,Minist Educ, Urumqi, Peoples R China
3.Beijing Univ Agr, Coll Plant Sci & Technol, Beijing, Peoples R China
关键词: Xinjiang wild walnut; JfDREB1A gene; Low-temperature stress; Transgenic Arabidopsis; Cold resistance
期刊名称:PEERJ ( 影响因子:3.061; 五年影响因子:3.537 )
ISSN: 2167-8359
年卷期: 2022 年 10 卷
页码:
收录情况: SCI
摘要: Background. Low-temperatures have the potential to be a serious problem for plants and can negatively affect the normal growth and development of walnuts. DREB1/CBF (Dehydration Responsive Element Binding Protein 1/C-repeat Binding Factor), one of the most direct transcription factors in response to low-temperature stress, may improve the resistance of plants to low-temperatures by regulating their functional genes. However, few studies have been conducted in walnut. The Xinjiang wild walnut is a rare wild plant found in China, with a large number of excellent trait genes, and is hardier than cultivated walnuts in Xinjiang.Methods. In this work, we identified all of the DREB1 members from the walnut genome and analyzed their expression levels in different tissues and during low -temperature stress on the Xinjiang wild walnut. The JfDREB1A gene of the Xinjiang wild walnut was cloned and transformed into Arabidopsis thaliana for functional verification.Results. There were five DREB1 transcription factors in the walnut genome. Among them, the relative expression level of the DREB1A gene was significantly higher than other members in the different tissues (root, stem, leaf) and was immediately un-regulated under low-temperature stress. The overexpression of the JfDREB1A gene increased the survival rates of transgenic Arabidopsis lines, mainly through maintaining the stability of cell membrane, decreasing the electrical conductivity and increasing the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the expression levels of cold-inducible genes like AtKIN1, AtERD10, AtRD29A, AtCOR15A and AtCOR47, were significantly increased. These results showed that the JfDREB1A gene may play an important role in the response to cold stress of the Xinjiang wild walnut. This study contributes to our understanding of the molecular mechanism of the Xinjiang wild walnut's response to low-temperature stress and will be beneficial for developing walnut cultivars with improved cold resistance.
- 相关文献
作者其他论文 更多>>
-
Super-pangenome analyses highlight genomic diversity and structural variation across wild and cultivated tomato species
作者:Li, Ning;Wang, Juan;Wang, Baike;Huang, Shaoyong;Yang, Tao;Tang, Yaping;Yang, Shengbao;Aisimutuola, Patiguli;Xu, Ruiqiang;Hu, Jiahui;Jia, Chunping;Ma, Kai;Yu, Qinghui;He, Qiang;Zhao, Jiantao;Zhou, Yongfeng;Zhang, Xinyan;Huang, Sanwen;Li, Hongbo;Huang, Shaoyong;Xu, Ruiqiang;Hu, Jiahui;Gao, Jie;Jia, Chunping;Lan, Haiyan;Li, Zhiqiang;Jiang, Fangling;Fei, Zhangjun;Fei, Zhangjun;Wang, Huan
关键词:
-
MdNAC104 positively regulates apple cold tolerance via CBF-dependent and CBF-independent pathways
作者:Mei, Chuang;Yang, Jie;Mei, Quanlin;Jia, Dongfeng;Gong, Xiaoqing;Guan, Qingmei;Mao, Ke;Ma, Fengwang;Mei, Chuang;Yan, Peng;Feng, Beibei;Mamat, Aisajan;Wang, Jixun
关键词:Malus domestica; Cold tolerance; MdNAC104; CBF pathway; Anthocyanin; ROS scavenging
-
Apple MdbHLH4 promotes the flowering transition through interactions with FLOWERING LOCUS C and transcriptional activation of FLOWERING LOCUS T
作者:Wang, Kangning;Liu, Huayu;Wang, Fei;Ma, Zhihang;Mei, Chuang;Ma, Fengwang;Mao, Ke;Mei, Chuang;Ma, Fengwang;Mao, Ke
关键词:Flowering; GA signaling; MdbHLH4; DELLA; MdFLC; MdFT
-
Potential roles of melatonin and ABA on apple dwarfing in semi-arid area of Xinjiang China
作者:Yan, Tianci;Song, Handong;Shan, Dongqian;Sun, Yanzhao;Hu, Zehui;Wang, Lin;Zhang, Tong;Kong, Jin;Yan, Tianci;Kong, Jin;Mei, Chuang;Wang, Jixun;Mei, Chuang;Wang, Jixun
关键词:Apple; Dwarfing; Melatonin (MT); Abscisic acid (ABA); Drought stress
-
Screening and functional prediction of differentially expressed genes in walnut endocarp during hardening period based on deep neural network under agricultural internet of things
作者:Guo, Zhongzhong;Yu, Shangqi;Guo, Zhongzhong;Yu, Shangqi;Fu, Jiazhi;Zhang, Rui;Guo, Zhongzhong;Yu, Shangqi;Fu, Jiazhi;Zhang, Rui;Fu, Jiazhi;Zhang, Rui;Ma, Kai
关键词:
-
Domestication and selection footprints in Persian walnuts (Juglans regia)
作者:Luo, Xiang;Luo, Xiang;Zhou, Huijuan;Yan, Feng;Chen, Pengpeng;Wang, Jiangtao;Ye, Hang;Li, Mengdi;Zhao, Peng;Luo, Xiang;Cao, Da;Qiao, Chengkui;Lu, Zhenhua;Cao, Shangying;Zhou, Huijuan;Zhou, Huijuan;Cao, Da;Liu, Chaobin;Woeste, Keith;Ebrahimi, Aziz;Chen, Xin;Fei, Zhangjun;An, Hong;Malvolti, Maria;Ma, Kai;Xu, Jiabao
关键词:
-
Analysis of metabolite differences in skin between Clapp's Favorite and its mutant Red Clapp's Favorite through non-targeted metabolomics
作者:Mu Hongmei;Ci Zhijuan;Liu Xiaohong;Du Xiaoyun;Yu Qiang;Li Qingyu;Li Yuanjun;Mamat, Aisajan;Lang Yanping
关键词:ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); plant metabolomics; differential metabolites; pear; fruit skin