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Bioinformatics Analysis, Expression Profiling, and Functional Characterization of Heat Shock Proteins in Wolfi-poria cocos

文献类型： 外文期刊

作者： Hu, Xin 1 ; Tang, Xue 1 ; Zhou, Yumei 1 ; Ahmad, Bilal 1 ; Zhang, Deli 4 ; Zeng, Yue 1 ; Wei, Jingyi 5 ; Deng, Liling 6 ; Chen, Shijiang 4 ; Pan, Yu 1 ;

作者机构： 1.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400715, Peoples R China

2.Key Lab Hort Sci Southern Mountainous Reg, Chongqing 400715, Peoples R China

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

4.Chongqing Acad Chinese Mat Med, Chongqing 400062, Peoples R China

5.Chongqing Acad Agr Sci, Chongqing 401329, Peoples R China

6.Chongqing Inst Biotechnol Co Ltd, Chongqing 401121, Peoples R China

关键词： Wolfiporia cocos; high temperature stress; heat shock protein; prokaryotic expression

期刊名称：BIOENGINEERING-BASEL （ 影响因子：4.6； ）

ISSN：

年卷期： 2023 年 10 卷 3 期

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) play critical roles in regulating different mechanisms under high-temperature conditions. HSPs have been identified and well-studied in different plants. However, there is a lack of information about their genomic organization and roles in medicinal plants and fungi, especially in Wolfi-poria cocos (W. cocos). We identified sixteen heat shock proteins (HSPs) in W. cocos and analyzed in terms of phylogenetic analysis, gene structure, motif distribution patterns, physiochemical properties, and expression comparison in different strains. Based on phylogenetic analysis, HSPs were divided into five subgroups (WcHSP100, WcHSP90, WcHSP70, WcHSP60, and WcsHSP). Subgroups WcHSP100s, WcHSP90s, WcHSP70s, WcHSP60, and WcsHSPs were further divided into 3, 2, 3, 1, and 6 subfamilies, respectively. Moreover, the expression profiling of all HSP genes in five strains of W. cocos under different temperature extremes revealed that expression of most HSPs were induced by high temperature. However, every subfamily showed different expression suggesting distinctive role in heat stress tolerance. WcHSP70-4, WcHSP90-1, and WcHSP100-1 showed the highest response to high temperature stress. Heterologous expression of WcHSP70-4, WcHSP90-1, and WcHSP100-1 genes in Escherichia coli enhanced survival rate of E. coli during heat stress. These findings suggest the role of W. cocos heat shock genes in the high temperature stress tolerance.

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